Labajova 2006 Gen Physiol Biophys: Difference between revisions
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|preparations=Permeabilized cells | |preparations=Permeabilized cells | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
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Revision as of 17:34, 25 February 2013
| LΓ‘bajovΓ‘ A, KofrΓ‘nek J, KrivΓ‘kovΓ‘ P, CervinkovΓ‘ Z, Drahota Z (2006) Tetraphenylphosphonium-Selective Electrode as a Tool for Evaluating Mitochondrial Permeability Transition Pore Function in Isolated Rat Hepatocytes. Gen Physiol Biophys 25: 325-331. |
Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z (2006) Gen Physiol Biophys
Abstract: The changes in mitochondrial membrane potential (ΞΟm) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca2+-induced ΞΟm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide. β’ Keywords: Tetraphenylphosphonium-selective electrode, Mitochondrial permeability transition pore, Hepatocytes
β’ O2k-Network Lab: CZ_Hradec Kralove_Cervinkova Z
Labels:
Organism: Rat
Tissue;cell: Liver
Preparation: Permeabilized cells
HRR: Oxygraph-2k
