Kuznetsov 2008 Nat Protoc
From Bioblast
Revision as of 10:30, 12 November 2010 by Wiethuechter Anita (talk | contribs)
Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat. Protoc. 3: 965-976. |
Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Nat. Protoc.
Abstract:
β’ O2k-Network Lab: EE_Tallinn_SaksV, FR_Grenoble_SaksV
Labels:
HRR: Oxygraph-2k
Corrections required
In Table 1, respiration of rat liver homogenate at 30 Β°C is actually mechanically permeabilized pig liver measured at 37 Β°C (Kuznetsov_2002_AnalytBiochem).
The protocol for permeabilized fibres lacks consideration on oxygen limitation (Kuznetsov_1998_BTK). Protocols are restricted to simple substrate supply (separate CI- or CII-electron entry into the ETS), whereas full OXPHOS capacity can be obtained only with physiological CI+II substrate combinations (Pesta_2010_Protocols).