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Difference between revisions of "Porter 2022 Abstract Bioblast"

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{{Abstract
{{Abstract
|title=Karavyraki M, Gnaiger E, Porter RK (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022.
|title=Karavyraki M, Gnaiger E, Porter RK (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference.
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference]
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference]
|authors=Karavyraki M, Gnaiger E, Porter RK
|authors=Karavyraki M, Gnaiger E, Porter RK
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::::#School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland.
::::#School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland.
::::#Oroboros Instruments, Innsbruck, Austria
::::#Oroboros Instruments, Innsbruck, Austria
::::Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 (2016-2021): MitoEAGLE.

Revision as of 15:10, 25 May 2022

Karavyraki M, Gnaiger E, Porter RK (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference.

Link: Bioblast 2022: BEC Inaugural Conference

Karavyraki M, Gnaiger E, Porter RK (2022)

Event: Bioblast 2022

In an endeavour to understand the metabolic phenotype behind metastasis from oral squamous cell carcinomas, we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial abundance but lower mitochondrial oxygen consumption rates than DOK cells. The lower oxygen consumption rate in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial abundance. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux.


β€’ Bioblast editor: Plangger M


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Affiliations and support

Marilena Karavyraki(1), Erich Gnaiger(2), Richard K. Porter(1)

  1. School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland.
  2. Oroboros Instruments, Innsbruck, Austria
Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 (2016-2021): MitoEAGLE.