Difference between revisions of "Jeger 2015 BioMed Res Internat"
Β |
|||
| (7 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Jeger V, Brandt S, Porta F, Jakob SM, Takala J, Djafarzadeh S (2015) Dose response of | |title=Jeger V, Brandt S, Porta F, Jakob SM, Takala J, Djafarzadeh S (2015) Dose response of Endotoxin on hepatocyte and muscle mitochondrial respiration ''in vitro''. BioMed Res Internat Article ID 353074:1-13. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25649304 PMID: 25649304 Open Acess] | |||
|authors=Jeger V, Brandt S, Porta F, Jakob SM, Takala J, Djafarzadeh S | |authors=Jeger V, Brandt S, Porta F, Jakob SM, Takala J, Djafarzadeh S | ||
|year=2015 | |year=2015 | ||
|journal= | |journal=BioMed Res Internat | ||
|abstract=Results on mitochondrial dysfunction in sepsis are controversial. We aimed to assess effects of LPS at wide dose and time ranges on hepatocytes and isolated skeletal muscle mitochondria. Human hepatocellular carcinoma cells (HepG2) | |abstract=Results on mitochondrial dysfunction in sepsis are controversial. We aimed to assess effects of LPS at wide dose and time ranges on hepatocytes and isolated skeletal muscle mitochondria. Human hepatocellular carcinoma cells (HepG2) | ||
were exposed to placebo or LPS (0.1, 1, and 10 πg/mL) for 4, 8, 16, and 24 hours and primary human hepatocytes to 1 πg/mL LPS or placebo (4, 8, and 16 hours). Mitochondria from porcine skeletal muscle samples were exposed to increasing doses of LPS (0.1β100 πg/mg) for 2 and 4 hours. Respiration rates of intact and permeabilized cells and isolated mitochondria were measured by | were exposed to placebo or LPS (0.1, 1, and 10 πg/mL) for 4, 8, 16, and 24 hours and primary human hepatocytes to 1 πg/mL LPS or placebo (4, 8, and 16 hours). Mitochondria from porcine skeletal muscle samples were exposed to increasing doses of LPS (0.1β100 πg/mg) for 2 and 4 hours. Respiration rates of intact and permeabilized cells and isolated mitochondria were measured by | ||
| Line 11: | Line 12: | ||
LPS reduced cellular ATP content of HepG2 cells, most likely as a result of the induced decrease in membrane potential. LPS decreased cellular and isolated mitochondrial respiration in a time-dependent, dose-dependent and complex-dependent manner. | LPS reduced cellular ATP content of HepG2 cells, most likely as a result of the induced decrease in membrane potential. LPS decreased cellular and isolated mitochondrial respiration in a time-dependent, dose-dependent and complex-dependent manner. | ||
|keywords=HepG2, Primary human hepatocytes | |||
|mipnetlab=CH Bern Djafarzadeh S | |mipnetlab=CH Bern Djafarzadeh S | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
|diseases=Sepsis | |||
|organism=Human | |organism=Human | ||
|tissues=Skeletal muscle, Liver | |tissues=Skeletal muscle, Liver, Other cell lines | ||
|preparations=Permeabilized cells, Isolated mitochondria | |preparations=Intact cells, Permeabilized cells, Isolated mitochondria | ||
|couplingstates=LEAK, ROUTINE, OXPHOS, ET | |||
|couplingstates=LEAK, ROUTINE, OXPHOS, | |pathways=N, S, CIV, NS, ROX | ||
| | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 14:46, 13 November 2017
| Jeger V, Brandt S, Porta F, Jakob SM, Takala J, Djafarzadeh S (2015) Dose response of Endotoxin on hepatocyte and muscle mitochondrial respiration in vitro. BioMed Res Internat Article ID 353074:1-13. |
Jeger V, Brandt S, Porta F, Jakob SM, Takala J, Djafarzadeh S (2015) BioMed Res Internat
Abstract: Results on mitochondrial dysfunction in sepsis are controversial. We aimed to assess effects of LPS at wide dose and time ranges on hepatocytes and isolated skeletal muscle mitochondria. Human hepatocellular carcinoma cells (HepG2) were exposed to placebo or LPS (0.1, 1, and 10 πg/mL) for 4, 8, 16, and 24 hours and primary human hepatocytes to 1 πg/mL LPS or placebo (4, 8, and 16 hours). Mitochondria from porcine skeletal muscle samples were exposed to increasing doses of LPS (0.1β100 πg/mg) for 2 and 4 hours. Respiration rates of intact and permeabilized cells and isolated mitochondria were measured by high-resolution respirometry.
In HepG2 cells, LPS reduced mitochondrial membrane potential and cellularATP content but did not modify basal respiration. Stimulated complex II respiration was reduced time-dependently using 1 πg/mL LPS. In primary human hepatocytes, stimulated mitochondrial complex II respiration was reduced time-dependently using 1 πg/mL LPS. In isolated porcine skeletal muscle mitochondria, stimulated respiration decreased at high doses (50 and 100 πg/mL LPS).
LPS reduced cellular ATP content of HepG2 cells, most likely as a result of the induced decrease in membrane potential. LPS decreased cellular and isolated mitochondrial respiration in a time-dependent, dose-dependent and complex-dependent manner. β’ Keywords: HepG2, Primary human hepatocytes
β’ O2k-Network Lab: CH Bern Djafarzadeh S
Labels: MiParea: Respiration
Pathology: Sepsis
Organism: Human Tissue;cell: Skeletal muscle, Liver, Other cell lines Preparation: Intact cells, Permeabilized cells, Isolated mitochondria
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k
