Difference between revisions of "Jarolim 2004 FEMS Yeast Res"
Bader Helga (talk | contribs) |
|||
| Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) A novel assay for replicative lifespan in Saccharomyces cerevisiae. FEMS Yeast Res 5: 169- | |title=Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) A novel assay for replicative lifespan in Saccharomyces cerevisiae. FEMS Yeast Res 5:169-77. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/15489200 PMID: 15489200] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/15489200 PMID: 15489200 Open Access] | ||
|authors=Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M | |authors=Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M | ||
|year=2004 | |year=2004 | ||
| Line 7: | Line 7: | ||
|abstract=The replicative lifespan of ''Saccharomyces cerevisiae'' is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations. | |abstract=The replicative lifespan of ''Saccharomyces cerevisiae'' is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations. | ||
|keywords=''Saccharomyces cerevisiae'', Yeast, Longevity, Aging, Replicative lifespan | |keywords=''Saccharomyces cerevisiae'', Yeast, Longevity, Aging, Replicative lifespan | ||
|mipnetlab= | |mipnetlab=AT Salzburg Breitenbach M | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Revision as of 15:15, 24 March 2015
| Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) A novel assay for replicative lifespan in Saccharomyces cerevisiae. FEMS Yeast Res 5:169-77. |
Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) FEMS Yeast Research
Abstract: The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations. • Keywords: Saccharomyces cerevisiae, Yeast, Longevity, Aging, Replicative lifespan
• O2k-Network Lab: AT Salzburg Breitenbach M
Labels:
Organism: Saccharomyces cerevisiae
Preparation: Intact cells
Coupling state: OXPHOS
HRR: Oxygraph-2k
