Gnaiger 2022 Abstract Bioblast-PB: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=[[File:Erich Gnaiger.jpg|left|100px|Erich Gnaiger]] Went N, | |title=[[File:Erich Gnaiger.jpg|left|100px|Erich Gnaiger]] Went N, Di Marcello M, <u>Gnaiger Erich</u> (2022) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by high-resolution PhotoRespirometry. Bioblast 2022: BEC Inaugural Conference. <br>[[Went 2021 MitoFit PB|Β»''MitoFit Preprint''Β«]] | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors=Went Nora, | |authors=Went Nora, Marcello M, Gnaiger Erich | ||
|year=2022 | |year=2022 | ||
|event=Bioblast 2022 | |event=Bioblast 2022 | ||
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== Affiliations and support == | == Affiliations and support == | ||
:::: Went | :::: Went N, Di Marcello M, Gnaiger Erich | ||
:::: | :::: Oroboros Instruments GmbH, Innsbruck, Austria | ||
:::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Unionβs Horizon 2020 research and innovation programme under grant agreement nΒΊ 859770 | :::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Unionβs Horizon 2020 research and innovation programme under grant agreement nΒΊ 859770. | ||
== List of abbreviations, terms and definitions - MitoPedia == | == List of abbreviations, terms and definitions - MitoPedia == |
Revision as of 10:01, 15 June 2022
Went N, Di Marcello M, Gnaiger Erich (2022) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by high-resolution PhotoRespirometry. Bioblast 2022: BEC Inaugural Conference. Β»MitoFit PreprintΒ« |
Link: Bioblast 2022: BEC Inaugural Conference
Went Nora, Marcello M, Gnaiger Erich (2022)
Event: Bioblast 2022
Algal biotechnology has emerged as a high-potential industry for efficient and CO2-neutral production of biomass providing biofuels, food and feed, and a variety of carbon-based chemicals and pharmaceuticals. Algal metabolism is directly involved in the regulation of growth, cell concentration, and biosynthesis of biotechnologically-relevant phytochemicals such as vitamins, antioxidants, and immune response boosters. Photoautotrophic growth rates of algae are based on light-to-chemical energy conversion and CO2 fixation, and any optimization of biomass production requires maximizing energy-use efficiency of photosynthesis and respiration, both of which vary as a function of light intensity. As such, the bioenergetic crosstalk between mitochondria and chloroplasts plays a key role in maintaining metabolic integrity and controlling intermediary metabolite production.
In the present study, we investigated how photosynthetic O2 production and respiratory O2 consumption was influenced as a function of light intensity, O2 concentration, and culture density in the unicellular model green alga Chlamydomonas reinhardtii. Cultures were grown photoautotrophically in a modified Tris-Phosphate growth medium (TRIS, N- and P-nutrient replete) at 25 Β°C, pH 7.0, and light intensity of 100 Β΅mol photonsΒ·s-1Β·m-2 (16:8 h light:dark cycle). Kinetics of light-induced O2 production and dark respiration of these microalgae was measured under culture conditions and three cell concentrations, while varying O2 concentrations in the Oroboros NextGen-O2k equipped with the PhotoBiology-Module [1] during stepwise increases of blue actinic light from from 10 to 350 Β΅molβs-1βm-2, followed by darkness, again at various controlled O2 concentrations. Maximum net photosynthesis was inhibited by 40 % at hyperoxic O2 concentrations of 550 to 650 Β΅M, when ROS production is known to be increased [2,3]. Transient light-enhanced dark respiration [4] peaked within 30 to 60 s after light-dark transitions and was 3.5- to 4-fold higher than steady-state dark respiration independent of O2 concentration in the range of 200 to 650 Β΅M.
We conclude that high-resolution photorespiratory analysis provides a new method to investigate the oxygen kinetics of O2 production and O2 consumption that reveal interactions of chloroplasts and mitochondria under precisely regulated experimental light and oxygen regimes.
- Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. https://doi.org/10.26124/mitofit:2021-0005
- KomlΓ³di T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004
- Shimakawa G, Kohara A, Miyake C (2020) Characterization of light-enhanced respiration in cyanobacteria. https://doi.org/10.3390/ijms22010342
β’ O2k-Network Lab: AT Innsbruck Oroboros
Affiliations and support
- Went N, Di Marcello M, Gnaiger Erich
- Oroboros Instruments GmbH, Innsbruck, Austria
- This work was part of the Oroboros NextGen-O2k project, with funding from the European Unionβs Horizon 2020 research and innovation programme under grant agreement nΒΊ 859770.
List of abbreviations, terms and definitions - MitoPedia
Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP
Organism: Plants
Preparation: Intact cells
Regulation: Oxygen kinetics Coupling state: ROUTINE
HRR: Oxygraph-2k, NextGen-O2k
Algae, LEDR, Photosynthesis