Garedew 2005 Eur Shock Soc: Difference between revisions
Purtschera (talk | contribs) No edit summary |
No edit summary |
||
| (29 intermediate revisions by 7 users not shown) | |||
| Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The | |title=Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The Oroboros O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11. | ||
|info=[ | |info=[[Media:Garedew 2005 Eur Shock Soc.pdf |'''Bioblast Access''']] | ||
|authors=Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen Duerr P, Gnaiger | |authors=Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger Erich | ||
|year=2005 | |year=2005 | ||
|abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the | |journal=Medimond | ||
Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[ | |abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the [[Oroboros O2k]], combining instrumental design, electronics and task-specific DatLab software. | ||
|mipnetlab= | Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP2k]] is applied for kinetics as a modular component of the O2k. [[DatLab]] provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information. | ||
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Jansen-Duerr P, AT Innsbruck Oroboros, AT Kolsass WGT | |||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |area=Respiration, Instruments;methods | ||
|organism=Human | |organism=Human | ||
|tissues=Fibroblast | |tissues=Fibroblast | ||
|preparations=Intact | |preparations=Intact cells | ||
| | |topics=Uncoupler | ||
| | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k, TIP2k | |||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Latest revision as of 04:16, 23 November 2021
| Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The Oroboros O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11. |
Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger Erich (2005) Medimond
Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the Oroboros O2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP2k is applied for kinetics as a modular component of the O2k. DatLab provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.
• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck Jansen-Duerr P, AT Innsbruck Oroboros, AT Kolsass WGT
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Tissue;cell: Fibroblast
Preparation: Intact cells
Regulation: Uncoupler Coupling state: OXPHOS
HRR: Oxygraph-2k, TIP2k
