Garedew 2005 Eur Shock Soc: Difference between revisions

Latest revision as of 04:16, 23 November 2021

Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The Oroboros O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11.

» Bioblast Access

Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger Erich (2005) Medimond

Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the Oroboros O2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP2k is applied for kinetics as a modular component of the O2k. DatLab provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.

• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck Jansen-Duerr P, AT Innsbruck Oroboros, AT Kolsass WGT

Labels: MiParea: Respiration, Instruments;methods

Organism: Human Tissue;cell: Fibroblast Preparation: Intact cells

Regulation: Uncoupler Coupling state: OXPHOS

HRR: Oxygraph-2k, TIP2k

@@ Line 1: / Line 1: @@
 {{Publication
-|title=Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11.
+|title=Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The Oroboros O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11.
 |info=[[Media:Garedew 2005 Eur Shock Soc.pdf |'''Bioblast Access''']]
-|authors=Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger E
+|authors=Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger Erich
 |year=2005
 |journal=Medimond
-|abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k|O2k]], combining instrumental design, electronics and task-specific DatLab software.
+|abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the [[Oroboros O2k]], combining instrumental design, electronics and task-specific DatLab software.
 Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP2k]] is applied for kinetics as a modular component of the O2k. [[DatLab]] provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.
-|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Jansen-Duerr P, AT Innsbruck OROBOROS
+|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Jansen-Duerr P, AT Innsbruck Oroboros, AT Kolsass WGT
 |discipline=Mitochondrial Physiology
 }}
@@ Line 20: / Line 20: @@
 |discipline=Mitochondrial Physiology
 }}
-== The present paper is fully quality controlled, but concerns emerged about the integrity of the first author ==
-:::* [http://retractionwatch.com/2012/09/04/three-retractions-for-university-college-london-mitochondrial-biologist-for-inappropriate-figure-manipulations/ University College London mitochondrial biologist resigns after three retractions for image fraud]
-== LinkedIn ==
-::: From '''https://www.linkedin.com/in/assegid/''' 2017-04-10
-:::: Assa Oumie - Currently Senior Analytical Development Scientist at Cell Therapy Catapult, growing the UK cell and gene therapy industry, delivering health and wealth. Previous to this I worked as a Lead Staff Scientist at Affymetrix UK for an NHS Diagnostic Lab-Academic Research Lab-CRO- Biotech collaborative R&D project, co-funded by the Innovate UK-Stratified Medicine Programme, to develop the OncoScan Assay for tumour genomic profiling.
-:::: As a Post Doctoral Researcher of Cell and Molecular Biology at University College London (UCL) I studied cell metabolism and identified therapeutic targets against inflammation and statin-induced myopathy, in collaboration with academic and industrial partners. During my career at UCL I secured funding from research council, charity and pharmaceutical industry for three major research projects, supervised the research projects of students and junior staff and delivered module courses to graduate students.
-:::: '''As a Business Development Associate and Post-Doctoral Researcher at Innsbruck Medical University (Austria) in partnership with a spin-out company, I played key roles in cell biology research, oxygen sensor and software development and validation, and customer technical support.''' Before moving to Innsbruck, I worked at the Medical Faculty of the University of Würzburg (Germany) where I conducted comparative metabolic investigation, on cancer and foetal cells, to better understand the mechanism of adaptation to hypoxia.
-::: '''Declaration'''
-:::: Assegid Garedew but not Assa Oumie (no person of that name is known to us) was a Postdoc employed by our 'spin-out company' working at the Medical University of Innsbruck (DSL Research Laboratory) after moving to Innsbruck from the University of Würzburg. ~ [[User:Gnaiger Erich|Gnaiger Erich]] ([[User talk:Gnaiger Erich|talk]]) 14:01, 10 April 2017 (CEST)