Difference between revisions of "Garedew 2005 Eur Shock Soc"
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|abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k]], combining instrumental design, electronics and task-specific DatLab software. | |abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k]], combining instrumental design, electronics and task-specific DatLab software. | ||
Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP2k]] is applied for kinetics as a modular component of the Oxygraph-2k. [[DatLab]] 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information. | Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP2k]] is applied for kinetics as a modular component of the Oxygraph-2k. [[DatLab]] 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information. | ||
|mipnetlab=AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen-Duerr P, AT Innsbruck | |mipnetlab=AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen-Duerr P, AT Innsbruck OROBOROS | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
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|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k, TIP2k | |instruments=Oxygraph-2k, TIP2k | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Revision as of 06:56, 16 August 2013
| Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS Oxygraph-2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna: 107-111. |
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Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger E (2005) Medimond
Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the OROBOROS Oxygraph-2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP2k is applied for kinetics as a modular component of the Oxygraph-2k. DatLab 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.
• O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen-Duerr P, AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Preparation: Intact cells
Regulation: Uncoupler Coupling state: OXPHOS
HRR: Oxygraph-2k, TIP2k
