Garedew 2005 Eur Shock Soc

Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS Oxygraph-2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna: 107-111.

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Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger E (2005) Medimond

Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the OROBOROS Oxygraph-2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP2k is applied for kinetics as a modular component of the Oxygraph-2k. DatLab 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.

• O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen-Duerr P, AT Innsbruck MitoCom

Labels: MiParea: Respiration, Instruments;methods 

Organism: Human 

Preparation: Intact cells 

Regulation: Uncoupler  Coupling state: OXPHOS 

HRR: Oxygraph-2k, TIP2k 

Instrumental and methodological aspects