Garcia-Poyatos 2024 Dev Cell
| García-Poyatos C, Arora P, Calvo E, Marques IJ, Kirschke N, Galardi-Castilla M, Lembke C, Meer M, Fernández-Montes P, Ernst A, Haberthür D, Hlushchuk R, Vázquez J, Vermathen P, Enríquez JA, Mercader N (2024) Cox7a1 controls skeletal muscle physiology and heart regeneration through complex IV dimerization. Dev Cell [Epub ahead of print]. https://doi.org/10.1016/j.devcel.2024.04.012 |
Garcia-Poyatos Carolina, Arora Prateek, Calvo Enrique, Marques Ines J, Kirschke Nick, Galardi-Castilla Maria, Lembke Carla, Meer Marco, Fernandez-Montes Paula, Ernst Alexander, Haberthuer David, Hlushchuk Ruslan, Vazquez Jesus, Vermathen Peter, Enriquez Jose Antonio, Mercader Nadia (2024) Dev Cell
Abstract: The oxidative phosphorylation (OXPHOS) system is intricately organized, with respiratory complexes forming super-assembled quaternary structures whose assembly mechanisms and physiological roles remain under investigation. Cox7a2l, also known as Scaf1, facilitates complex III and complex IV (CIII-CIV) super-assembly, enhancing energetic efficiency in various species. We examined the role of Cox7a1, another Cox7a family member, in supercomplex assembly and muscle physiology. Zebrafish lacking Cox7a1 exhibited reduced CIV2 formation, metabolic alterations, and non-pathological muscle performance decline. Additionally, cox7a1-/- hearts displayed a pro-regenerative metabolic profile, impacting cardiac regenerative response. The distinct phenotypic effects of cox7a1-/- and cox7a2l-/- underscore the diverse metabolic and physiological consequences of impaired supercomplex formation, emphasizing the significance of Cox7a1 in muscle maturation within the OXPHOS system. • Keywords: Ca(2+) signaling, cox7a1, cox7a2l, Electron transport chain, Heart regeneration, Metabolic rewiring, Mitochondria, Muscle physiology, scaf1, Supercomplex assembly, Zebrafish • Bioblast editor: Plangger M • O2k-Network Lab: ES Madrid Enriquez JA
Labels: MiParea: Respiration, Genetic knockout;overexpression
Organism: Zebrafish
Tissue;cell: Skeletal muscle
Preparation: Isolated mitochondria
Enzyme: Complex III, Complex IV;cytochrome c oxidase
Coupling state: LEAK, OXPHOS Pathway: N, S, CIV, NS, ROX HRR: Oxygraph-2k
2024-05
