Garcia-Neto 2017 PLOS ONE: Difference between revisions
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{{Publication | {{Publication | ||
|title=Garcia-Neto W, Cabrera-Orefice A, Uribe-Carvajal S, Kowaltowski AJ, | |title=Garcia-Neto W, Cabrera-Orefice A, Uribe-Carvajal S, Kowaltowski AJ, LuΓ©vano-MartΓnez LA (2017) High osmolarity environments activate the mitochondrial alternative oxidase in ''Debaryomyces Hansenii''. PLOS ONE 12:e0169621. https://doi.org/10.1371/journal.pone.0169621 | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/28060946 PMID: 28060946 Open Access] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/28060946 PMID: 28060946 Open Access] | ||
|authors=Garcia-Neto W, Cabrera-Orefice A, Uribe-Carvajal S, Kowaltowski AJ, Luevano-Martinez AL | |authors=Garcia-Neto W, Cabrera-Orefice A, Uribe-Carvajal S, Kowaltowski AJ, Luevano-Martinez AL | ||
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|keywords=Alternative oxidase | |keywords=Alternative oxidase | ||
|editor=[[Kandolf G]], | |editor=[[Kandolf G]], | ||
|mipnetlab=BR Sao Paulo Kowaltowski AJ | |mipnetlab=BR Sao Paulo Kowaltowski AJ, MX Mexico City Uribe-Carvajal S | ||
}} | }} | ||
[[File:Garcia-Neto 2017 PLOS ONE CORRECTION.png|right|400px]] | |||
{{Template:Correction FADH2 and S-pathway}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP | |area=Respiration, Comparative MiP;environmental MiP | ||
|organism=Fungi | |organism=Fungi | ||
|preparations=Intact cells, Isolated mitochondria | |preparations=Intact cells, Isolated mitochondria | ||
|couplingstates=ROUTINE | |couplingstates=ROUTINE | ||
|pathways=CIV, ROX | |pathways=CIV, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Latest revision as of 08:14, 19 November 2023
Garcia-Neto W, Cabrera-Orefice A, Uribe-Carvajal S, Kowaltowski AJ, LuΓ©vano-MartΓnez LA (2017) High osmolarity environments activate the mitochondrial alternative oxidase in Debaryomyces Hansenii. PLOS ONE 12:e0169621. https://doi.org/10.1371/journal.pone.0169621 |
Garcia-Neto W, Cabrera-Orefice A, Uribe-Carvajal S, Kowaltowski AJ, Luevano-Martinez AL (2017) PLOS ONE
Abstract: The oleaginous yeast Debaryomyces hansenii is a good model to understand molecular mechanisms involved in halotolerance because of its impressive ability to survive under a wide range of salt concentrations. Several cellular adaptations are implicated in this response, including the presence of a cyanide-insensitive ubiquinol oxidase (Aox). This protein, which is present in several taxonomical orders, has been related to different stress responses. However, little is known about its role in mitochondria during transitions from low to high saline environments. In this report, we analyze the effects of Aox in shifts from low to high salt concentrations in the culture media. At early stages of a salt insult, we observed that this protein prevents the overflow of electrons on the mitochondrial respiratory chain, thus, decreasing the production of reactive oxygen species. Interestingly, in the presence of high osmolite concentrations, Aox activity is able to sustain a stable membrane potential when coupled to complex I, despite a compromised cytochrome pathway. Taken together, our results suggest that under high osmolarity conditions Aox plays a critical role regulating mitochondrial physiology. β’ Keywords: Alternative oxidase β’ Bioblast editor: Kandolf G β’ O2k-Network Lab: BR Sao Paulo Kowaltowski AJ, MX Mexico City Uribe-Carvajal S
Correction: FADH2 and Complex II
- FADH2 is shown as the substrate feeding electrons into Complex II (CII). This is wrong and requires correction - for details see Gnaiger (2024).
- Gnaiger E (2024) Complex II ambiguities β FADH2 in the electron transfer system. J Biol Chem 300:105470. https://doi.org/10.1016/j.jbc.2023.105470 - Β»Bioblast linkΒ«
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Organism: Fungi
Preparation: Intact cells, Isolated mitochondria
Coupling state: ROUTINE
Pathway: CIV, ROX
HRR: Oxygraph-2k