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Flux control ratio

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Revision as of 13:16, 6 August 2019 by Antunes Diana (talk | contribs)


high-resolution terminology - matching measurements at high-resolution


Flux control ratio

Description

Flux control ratios (FCR), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%).

For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation with sequential titrations (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.

Abbreviation: FCR

Reference: Gnaiger 2014 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Pesta 2012 Methods Mol Biol, MiPNet19.18E O2 flux analysis - DatLab 6


MitoPedia concepts: Respiratory control ratio, SUIT concept 


MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: DatLab 

Unknown sample concentration and normalization per unit sample [x]

  • Used in new Excel Template for DatLab 7.4
If the sample concentration is not yet known, the box ‘Known sample concentration’ can be unchecked, and the concentration will be considered by default as 1, with units [x·mL-1]. In this way, flux values can be normalized and FCRs can be obtained even if the sample concentration is unknown. Extensive_quantity


Flux control factor: normalization of mitochondrial respiration

» More details: Flux control factor

DatLab

The entire plot of oxygen flux can be transformed to a FCR. Click on 'Flux/Slope' in the pull-down menu. - MiPNet19.18E O2 flux analysis - DatLab 6.