Fang 2010 Cell: Difference between revisions
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|area=Respiration | |||
|preparations=Intact cells | |preparations=Intact cells | ||
|injuries=Anaerobic metabolism, Cancer; Apoptosis; Cytochrome c | |injuries=Anaerobic metabolism, Cancer; Apoptosis; Cytochrome c | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k |
Revision as of 12:04, 9 August 2013
Fang M, Shen Z, Huang S, Zhao L, Chen S, Mak TW, Wang X (2010) The ER UDPase ENTPD5 promotes protein N-glycosylation, the Warburg effect, and proliferation in the PTEN pathway. Cell 143: 711-724. |
Fang M, Shen Z, Huang S, Zhao L, Chen S, Mak TW, Wang X (2010) Cell
Abstract: PI3K and PTEN lipid phosphatase control the level of cellular phosphatidylinositol (3,4,5)-trisphosphate, an activator of AKT kinases that promotes cell growth and survival. Mutations activating AKT are commonly observed in human cancers. We report here that ENTPD5, an endoplasmic reticulum (ER) enzyme, is upregulated in cell lines and primary human tumor samples with active AKT. ENTPD5 hydrolyzes UDP to UMP to promote protein N-glycosylation and folding in ER. Knockdown of ENTPD5 in PTEN null cells causes ER stress and loss of growth factor receptors. ENTPD5, together with cytidine monophosphate kinase-1 and adenylate kinase-1, constitute an ATP hydrolysis cycle that converts ATP to AMP, resulting in a compensatory increase in aerobic glycolysis known as the Warburg effect. The growth of PTEN null cells is inhibited both in vitro and in mouse xenograft tumor models. ENTPD5 is therefore an integral part of the PI3K/PTEN regulatory loop and a potential target for anticancer therapy โข Keywords: Warburg effect
โข O2k-Network Lab: US_TX Dallas_Wang X, CN_Beijing_Wang X
Labels: MiParea: Respiration
Stress:Anaerobic metabolism"Anaerobic metabolism" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property., Cancer; Apoptosis; Cytochrome c"Cancer; Apoptosis; Cytochrome c" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.
Preparation: Intact cells
Coupling state: OXPHOS
HRR: Oxygraph-2k
Oxygraph-2k application
In this publication and in Supplemental Information (EXTENDED EXPERIMENTAL PROCEDURES) respirometric results on intact cells are described which are based on applications of the Oroboros Oxygraph-2k (Dr. Huang Song, personal communication).