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| Attention! Attention!
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| Disclaimer: This is an alpha version and page is a currently still under development. Please DO NOT consider information below in this page to be true or of any scientific value.
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| This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.
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| {| class="wikitable"
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| ! Protocol name
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| ! Step 1
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| ! Step 2
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| ! Step 3
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| ! Step 4
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| ! Step 5
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| ! Step 6
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| ! Step 7
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| ! Step 8
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| ! Step 9
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| ! Step 10
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| ! Step 11
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| ! Step 12
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| ! Step 13
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| ! Step 14
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| ! Step 15
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| ! Step 16
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| ! Step 17
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| ! Step 18
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| ! Step 19
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| ! Step 20
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| ! Short name
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| ! Notes
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| | SUIT-001_O2_ce-pce_D003
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| | kajsd
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| | alsdkjlk
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| | kasdjlak
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| | koasidaosid
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| | lkjahsdhvcb
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| | RP1
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| | Doerrier 2018 Methods Mol Biol
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| | SUIT-001_O2_PBMC-PLT_D004
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| | jasdkdjadkjdakjasd
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| | adsjlaks
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| | sdada
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| | asdad
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| | asddadaa
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| | RP1
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| | Doerrier 2018 Methods Mol Biol
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| |-
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| | SUIT-001_O2_mt_D001
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| | 1mt
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| | 1PM
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| | 2D
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| | 2c
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| | 3U
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| | 4G
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| | 5S
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| | 6Oct
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| | 7Rot
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| | 8Gp
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| | 9Ama
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| | 10AsTm
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| | 11Azd
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| | RP1
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| | Doerrier 2018 Methods Mol Biol
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| | SUIT-001_O2_pfi_D002
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| | RP1
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| | Doerrier 2018 Methods Mol Biol
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| | SUIT-002_O2_ce-pce-PBMC-PLT_D007a
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| | RP2
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| | A: Doerrier 2018 Methods Mol Biol
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| | SUIT-002_O2_ce-pce_D007
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| | RP2
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| | A: Doerrier 2018 Methods Mol Biol
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| | SUIT-002_O2_mt_D005
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| | RP2
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| | A: Doerrier 2018 Methods Mol Biol
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| | SUIT-002_O2_pfi_D006
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| | RP2
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| | A: Doerrier 2018 Methods Mol Biol
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| | SUIT-003_AmR_ce_D017
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_AmR_ce_D058
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_AmR_ce_D059
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce-pce_D013
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce-pce_D018
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce-pce_D020
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D009
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D012
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D028
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D037
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D038
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D039
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D050
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D060
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D061
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-003_O2_ce_D062
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| | CCP-ce
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| | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
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| | SUIT-004_O2_pfi_D010
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| | RP1-short
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| | SUIT-005_O2_pfi_D011
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| | RP2-short
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| | A: when malate-anaplerotic activity is zero
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| | SUIT-006_AmR_mt_D048
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| | CCP-mtprep
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| | A: Coupling control protocol, mtprep
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| | SUIT-006_Fluo_mt_D034
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| | CCP-mtprep
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| | A: Coupling control protocol, mtprep
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| | SUIT-006_Fluo_mt_D034_chemical background
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| | CCP-mtprep
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| | A: Coupling control protocol, mtprep
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| | SUIT-006_MgG_mt_D055
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| | CCP-mtprep
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| | A: Coupling control protocol, mtprep
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| | SUIT-006_O2_ce-pce_D029
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| | CCP-mtprep
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| | A: Coupling control protocol, mtprep
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| | SUIT-006_O2_mt_D022
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| | CCP-mtprep
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| | A: Coupling control protocol, mtprep
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| | SUIT-006_O2_mt_D047
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| | CCP-mtprep
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| | A: Coupling control protocol, mtprep
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| | SUIT-007_O2_ce-pce_D030
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| | Glutamate anaplerosis
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| | A: Glutamate anaplerotic pathway
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| | SUIT-008_O2_ce-pce_D025
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| | PM+G+S_OXPHOS+Rot_ET
| |
| | A: Additivity between the N- and S-pathway in the Q-junction
| |
| |-
| |
| | SUIT-008_O2_mt_D026
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| |
| | PM+G+S_OXPHOS+Rot_ET
| |
| | A: Additivity between the N- and S-pathway in the Q-junction
| |
| |-
| |
| | SUIT-008_O2_pfi_D014
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| |
| | PM+G+S_OXPHOS+Rot_ET
| |
| | A: Additivity between the N- and S-pathway in the Q-junction
| |
| |-
| |
| | SUIT-009_AmR_ce-pce_D019
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| |
| |
| |-
| |
| | SUIT-009_AmR_mt_D021
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| |
| |-
| |
| | SUIT-009_O2_ce-pce_D016
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| |
| |-
| |
| | SUIT-009_O2_mt_D015
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| |
| |
| |
| |-
| |
| | SUIT-010_O2_ce-pce_D008
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| |
| |
| | Digitonin test
| |
| | A: Optimization of digitonin concentration for pce
| |
| |-
| |
| | SUIT-011_O2_pfi_D024
| |
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| |
| |
| | GM+S_OXPHOS+Rot_ET
| |
| | A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control
| |
| |-
| |
| | SUIT-012_O2_ce-pce_D052
| |
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| |
| | PM+G_OXPHOS
| |
| | A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
| |
| |-
| |
| | SUIT-012_O2_mt_D027
| |
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| |
| |
| | PM+G_OXPHOS
| |
| | A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
| |
| |-
| |
| | SUIT-013_AmR_ce_D023
| |
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| |
| |
| |
| |
| |
| |
| |
| |-
| |
| | SUIT-014_O2_pfi_D042
| |
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| |
| |
| | GM+P+S_OXPHOS+Rot_ET
| |
| | A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
| |
| |-
| |
| | SUIT-015_O2_pti_D043
| |
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| |
| |
| | F+G+P+S_OXPHOS+Rot_ET
| |
| | A: F-pathway in LEAK state and OXPHOS state
| |
| |-
| |
| | SUIT-016_O2_pfi_D044
| |
| |
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| |
| |
| | F+G+S+Rot_OXPHOS+Omy
| |
| | A: F-pathway in LEAK state and OXPHOS state
| |
| |-
| |
| | SUIT-017_O2_mt _D046
| |
| |
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| |
| |
| | F+G+S_OXPHOS+Rot_ET
| |
| | A:
| |
| |-
| |
| | SUIT-017_O2_pfi_D049
| |
| |
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| |
| |
| |
| | F+G+S_OXPHOS+Rot_ET
| |
| | A:
| |
| |-
| |
| | SUIT-018_AmR_mt_D031
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| | SUIT-018_AmR_mt_D040
| |
| |
| |
| |
| |
| |
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| |
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| |
| |
| |
| |
| |
| |
| |
| |-
| |
| | SUIT-018_AmR_mt_D041
| |
| |
| |
| |
| |
| |
| |
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| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| | SUIT-018_O2_mt_D054
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| | SUIT-019_O2_pfi_D045
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| | Pal+Oct+P+G_OXPHOS+S+Rot_ET
| |
| | A:
| |
| |-
| |
| | SUIT-020_Fluo_mt_D033
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| | PM+G+S+Rot_OXPHOS+Omy
| |
| | A: simultaneous determination of O2 flux and mt-membrane potential
| |
| |-
| |
| | SUIT-020_Fluo_mt_D033_chemical_background
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| | PM+G+S+Rot_OXPHOS+Omy
| |
| | A: simultaneous determination of O2 flux and mt-membrane potential
| |
| |-
| |
| | SUIT-020_O2_mt_D032
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| | PM+G+S+Rot_OXPHOS+Omy
| |
| | A: simultaneous determination of O2 flux and mt-membrane potential
| |
| |-
| |
| | SUIT-021_Fluo_mt_D036
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| | OXPHOS (GM+S+Rot+Omy)
| |
| | A: simultaneous determination of O2 flux and mt-membrane potential
| |
| |-
| |
| | SUIT-021_Fluo_mt_D036_chemical background
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| | OXPHOS (GM+S+Rot+Omy)
| |
| | A: simultaneous determination of O2 flux and mt-membrane potential
| |
| |-
| |
| | SUIT-021_O2_mt_D035
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| | OXPHOS (GM+S+Rot+Omy)
| |
| | A: simultaneous determination of O2 flux and mt-membrane potential
| |
| |-
| |
| | SUIT-022 O2 ce D051
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| | AOX (ce CN+SHAM)
| |
| | A: Determination of the respiration due to the alternative oxidase pathway
| |
| |-
| |
| | SUIT-023 O2 ce D053
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| | AOX-ce SHAM+CN
| |
| | A: Determination of respiration through the CIII-CIV pathway
| |
| |-
| |
| | SUIT-024_O2_ce-pce_D056
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| | ATPase (PM)
| |
| | A: Determination of the presence of ATPases in mitochondrial preparations.
| |
| |-
| |
| | SUIT-025_O2_mt_D057
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| | OXPHOS (F+M+P+G+S+Rot)
| |
| |
| |
| |-
| |
| | SUIT-026_AmR_mt_D064
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| | SUIT-026_O2_mt_D063
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| | SUIT-027_O2_ce-pce_D065
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
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| |
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| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| | Malate anaplerosis
| |
| | A: Malate anaplerotic pathway
| |
| |}
| |