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Difference between revisions of "Excel templ: Protocols"

From Bioblast
Line 4: Line 4:


This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.
This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.
{| class="wikitable"
{| class="wikitable"
! Protocol name
! Protocol name
! D number
! Step 1
! Step 1
! Step 2
! Step 2
Line 26: Line 28:
! Step 19
! Step 19
! Step 20
! Step 20
! Short name
! Number of steps
! Notes
|-
|-
| SUIT-001_O2_ce-pce_D003
| SUIT-001_O2_ce-pce_D003
| kajsd
| D003
| alsdkjlk
| ce1
| kasdjlak
| 1Dig
| koasidaosid
| 1PM
| lkjahsdhvcb
| 2D
|  
| 2c
| asdda
| 3U
|  
| 4G
| adsasda
| 5S
|  
| 6Oct
|  
| 7Rot
|  
| 8Gp
| 9Ama
| 10AsTm
| 11Azd
|  
|  
|  
|  
Line 48: Line 52:
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| 14
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| RP1
| Doerrier 2018 Methods Mol Biol
|-
|-
| SUIT-001_O2_PBMC-PLT_D004
| SUIT-001_O2_PBMC-PLT_D004
| jasdkdjadkjdakjasd
| D004
| adsjlaks
| ce1
| sdada
| 1Dig
| asdad
| 1PM
| asddadaa
| 2D
| 2c
| 3U
| 4G
| 5S
| (6Oct)
| 7Rot
| 8Gp
| 9Ama
| 10AsTm
| 11Azd
|  
|  
|  
|  
Line 65: Line 76:
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| 14
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| RP1
| Doerrier 2018 Methods Mol Biol
|-
|-
| SUIT-001_O2_mt_D001
| SUIT-001_O2_mt_D001
| 1mt
| D001
| 1PM
| 1PM
| 2D
| 2D
Line 98: Line 99:
|  
|  
|  
|  
| RP1
|  
| Doerrier 2018 Methods Mol Biol
| 12
|-
|-
| SUIT-001_O2_pfi_D002
| SUIT-001_O2_pfi_D002
| D002
| 1PM
| 2D
| 2c
| 3U
| 4G
| 5S
| 6Oct
| 7Rot
| 8Gp
| 9Ama
| 10AsTm
| 11Azd
|  
|  
|  
|  
Line 110: Line 124:
|  
|  
|  
|  
| 12
|-
| SUIT-002_O2_ce-pce_D007
| D007
| ce1
| 1Dig
| 1D
| 2M
| 4Oct
| 3c
| 4M
| 5P
| 6G
| 7S
| 8Gp
| 9U
| 10Rot
| 11Ama
| 12AsTm
| 13Azd
|  
|  
|  
|  
|  
|  
|  
|  
|  
| 16
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|
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|
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| RP1
| Doerrier 2018 Methods Mol Biol
|-
|-
| SUIT-002_O2_ce-pce-PBMC-PLT_D007a
| SUIT-002_O2_ce-pce-PBMC-PLT_D007a
| D007a
| ce1
| 1Dig
| 1D
| 2M
| 4Oct
| 3c
| 4M
| 5P
| 6G
| 7S
| 8Gp
| 9U
| 10Rot
| 11Ama
| 12AsTm
| 13Azd
|  
|  
|  
|  
|  
|  
|  
|  
|  
| 16
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| RP2
| A: Doerrier 2018 Methods Mol Biol
|-
| SUIT-002_O2_ce-pce_D007
|
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| RP2
| A: Doerrier 2018 Methods Mol Biol
|-
|-
| SUIT-002_O2_mt_D005
| SUIT-002_O2_mt_D005
| D005
| 1D
| 2M
| 3Oct
| 3c
| 4M
| 5P
| 6G
| 7S
| 8Gp
| 9U
| 10Rot
| 11Ama
| 12AsTm
| 13Azd
|  
|  
|  
|  
Line 180: Line 196:
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| 14
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| RP2
| A: Doerrier 2018 Methods Mol Biol
|-
|-
| SUIT-002_O2_pfi_D006
| SUIT-002_O2_pfi_D006
| D006
| 1D
| 2M
| 3Oct
| 3c
| 4M
| 5P
| 6G
| 7S
| 8Gp
| 9U
| 10Rot
| 11Ama
| 12AsTm
| 13Azd
|  
|  
|  
|  
Line 204: Line 220:
|  
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| 14
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| RP2
| A: Doerrier 2018 Methods Mol Biol
|-
|-
| SUIT-003_AmR_ce_D017
| SUIT-003_AmR_ce_D017
| D017
| ce1
| ce2P
| ce3Omy
| ce4U
| ce5Rot
| ce6S
| ce7Ama
|  
|  
|  
|  
Line 235: Line 244:
|  
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| 7
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_AmR_ce_D058
| SUIT-003_AmR_ce_D058
| D058
| ce1
| ce1SOD
| ce1HRP
| ce1AmR
| ce2Omy
| ce3U
| ce4Rot
| ce5Ama
|  
|  
|  
|  
Line 258: Line 268:
|  
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|  
|  
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| 8
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_AmR_ce_D059
| SUIT-003_AmR_ce_D059
| D059
| ce1
| ce1H2O
| ce1MiR05
| ce1DMSO
| ce2Omy
| ce3U
| ce4Rot
| ce5Ama
|  
|  
|  
|  
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| 8
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
| SUIT-003_O2_ce-pce_D013
|
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
| SUIT-003_O2_ce-pce_D018
|
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
| SUIT-003_O2_ce-pce_D020
|
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D009
| SUIT-003_O2_ce_D009
| D009
| ce1
| ce2Omy
| ce3U
| ce4Rot
| ce5Ama
|  
|  
|  
|  
Line 381: Line 316:
|  
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| 5
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D012
| SUIT-003_O2_ce_D012
| D012
| ce1
| ce1P
| ce2Omy
| ce3U
| ce4Rot
| ce5Ama
|  
|  
|  
|  
Line 404: Line 340:
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|  
|  
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| 6
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D028
| SUIT-003_O2_ce_D028
| D028
| ce1
| ce2Omy
| ce3U
| ce4Rot
| ce5S
| ce6Ama
|  
|  
|  
|  
Line 428: Line 364:
|  
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|  
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| 6
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D037
| SUIT-003_O2_ce_D037
| D037
| ce1
| ce1Glc
| (ce2Omy)
| ce3U
| ce4Ama
|  
|  
|  
|  
Line 453: Line 388:
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| 5
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D038
| SUIT-003_O2_ce_D038
| D038
| ce1
| (ce2Omy)
| ce3U
| ce3Glc
| ce3'U
| ce4Ama
|  
|  
|  
|  
Line 476: Line 412:
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| 6
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D039
| SUIT-003_O2_ce_D039
| D039
| ce1
| (ce2Omy)
| ce3U
| ce4Rot
| ce5Ama
|  
|  
|  
|  
Line 501: Line 436:
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|  
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|  
| 5
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D050
| SUIT-003_O2_ce_D050
| D050
| ce1
| ce1Snv
| (ce2Omy)
| ce3U
| ce4Rot
| ce5Ama
|  
|  
|  
|  
Line 524: Line 460:
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| 6
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D060
| SUIT-003_O2_ce_D060
| D060
| ce1
| (ce2Omy)
| ce3U
| ce4Rot
| ce5Snv
| ce6Mnanv
| ce7Ama
|  
|  
|  
|  
Line 547: Line 484:
|  
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|  
|  
|  
| 7
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D061
| SUIT-003_O2_ce_D061
| D061
| ce1
| (ce2Omy)
| ce3U
| ce4Rot
| ce5DMSO
| ce6DMSO
| ce7Ama
|  
|  
|  
|  
Line 571: Line 508:
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| 7
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| CCP-ce
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-003_O2_ce_D062
| SUIT-003_O2_ce_D062
| D062
| ce1
| ce1DMSO
| (ce2Omy)
| ce3U
| ce4Rot
| ce5Ama
|  
|  
|  
|  
Line 596: Line 532:
|  
|  
|  
|  
| 6
|-
| SUIT-003_O2_ce-pce_D013
| D013
| ce1
| ce1P
| ce2Omy
| ce3U
| ce3Glc
| ce3M
| ce4Rot
| ce5S
| 1Dig
| 1U
| 1c
| 2Ama
| 3AsTm
| 4Azd
|  
|  
|  
|  
Line 602: Line 556:
|  
|  
|  
|  
| CCP-ce
| 14
| A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
|-
|-
| SUIT-004_O2_pfi_D010
| SUIT-003_O2_ce-pce_D018
| D018
| ce1
| ce1P
| ce2Omy
| ce3U
| ce3Glc
| ce4Rot
| ce5S
| 1Dig
| 1c
| 2Ama
| 3AsTm
| 4Azd
|  
|  
|  
|  
Line 614: Line 580:
|  
|  
|  
|  
| 12
|-
| SUIT-003_O2_ce-pce_D020
| D020
| ce1
| ce1P
| ce2Omy
| ce3U
| ce4Rot
| ce5S
| 1Dig
| 1c
| 2Ama
| 3AsTm
| 4Azd
|  
|  
|  
|  
Line 623: Line 604:
|  
|  
|  
|  
| 11
|-
| SUIT-004_O2_pfi_D010
| D010
| 1PM
| 2D
| 2c
| 3U
| 4S
| 5Rot
| 6Ama
| 7AsTm
| 8Azd
|  
|  
|  
|  
|  
|  
| RP1-short
|  
|  
|-
| SUIT-005_O2_pfi_D011
|  
|  
|  
|  
Line 637: Line 628:
|  
|  
|  
|  
| 9
|-
| SUIT-005_O2_pfi_D011
| D011
| 1OctM
| 2D
| 3P
| 4S
| 5U
| 6Rot
| 7Ama
| 8AsTm
| 9Azd
|  
|  
|  
|  
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| 9
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| RP2-short
| A: when malate-anaplerotic activity is zero
|-
|-
| SUIT-006_AmR_mt_D048
| SUIT-006_AmR_mt_D048
| D048
| 1PM
| 2D
| 3Omy
| 4U
| 5Ama
|  
|  
|  
|  
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| 5
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| CCP-mtprep
| A: Coupling control protocol, mtprep
|-
|-
| SUIT-006_Fluo_mt_D034
| SUIT-006_Fluo_mt_D034
| D034
| 1PM
| 2D
| 3Omy
| 4U
| 5Ama
|  
|  
|  
|  
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| 5
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| CCP-mtprep
| A: Coupling control protocol, mtprep
|-
| SUIT-006_Fluo_mt_D034_chemical background
|
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| CCP-mtprep
| A: Coupling control protocol, mtprep
|-
|-
| SUIT-006_MgG_mt_D055
| SUIT-006_MgG_mt_D055
| D055
| 1PM
| 2D
| 3Cat
| 4U
| 5Ama
|  
|  
|  
|  
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| 5
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| CCP-mtprep
| A: Coupling control protocol, mtprep
|-
|-
| SUIT-006_O2_ce-pce_D029
| SUIT-006_O2_ce-pce_D029
| D029
| ce1
| 1Dig
| 1PM
| 2D
| 2c
| 3Omy
| 4U
| 5Ama
|  
|  
|  
|  
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| 8
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| CCP-mtprep
| A: Coupling control protocol, mtprep
|-
|-
| SUIT-006_O2_mt_D022
| SUIT-006_O2_mt_D022
| D022
| 1SRot
| 2D
| 2c
| (3Omy)
| 4U
| 5Ama
|  
|  
|  
|  
Line 788: Line 772:
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| 6
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| CCP-mtprep
| A: Coupling control protocol, mtprep
|-
|-
| SUIT-006_O2_mt_D047
| SUIT-006_O2_mt_D047
| D047
| 1PM
| 2D
| 2c
| 3Omy
| 4U
| 5Ama
|  
|  
|  
|  
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| 6
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| CCP-mtprep
| A: Coupling control protocol, mtprep
|-
|-
| SUIT-007_O2_ce-pce_D030
| SUIT-007_O2_ce-pce_D030
| D030
| ce1
| 1Dig
| 1G
| 2D
| 2c
| 3M
| 4U
| 5Ama
|  
|  
|  
|  
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| 8
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|
| Glutamate anaplerosis
| A: Glutamate anaplerotic pathway
|-
|-
| SUIT-008_O2_ce-pce_D025
| SUIT-008_O2_ce-pce_D025
| D025
| ce1
| 1Dig
| 1PM
| 2D
| 2c
| 3G
| 4S
| 5U
| 6Rot
| 7Ama
| 8AsTm
| 9Azd
|  
|  
|  
|  
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| 12
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|
| PM+G+S_OXPHOS+Rot_ET
| A: Additivity between the N- and  S-pathway in the Q-junction
|-
|-
| SUIT-008_O2_mt_D026
| SUIT-008_O2_mt_D026
| D026
| 1PM
| 2D
| 2c
| 3G
| 4S
| 4D
| 5U
| 6Rot
| 7Ama
| 8AsTm
| 9Azd
|  
|  
|  
|  
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| 11
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| PM+G+S_OXPHOS+Rot_ET
| A: Additivity between the N- and  S-pathway in the Q-junction
|-
|-
| SUIT-008_O2_pfi_D014
| SUIT-008_O2_pfi_D014
| D014
| 1PM
| 2D
| 2c
| 3G
| 4S
| 4D
| 5U
| 6Rot
| 7Ama
| 8AsTm
| 9Azd
|  
|  
|  
|  
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| 11
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| PM+G+S_OXPHOS+Rot_ET
| A: Additivity between the N- and  S-pathway in the Q-junction
|-
|-
| SUIT-009_AmR_ce-pce_D019
| SUIT-009_AmR_ce-pce_D019
| D019
| ce1
| 1Dig
| 1S
| 2D
| 3P
| 4Rot
| 5Ama
|  
|  
|  
|  
Line 931: Line 916:
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| 7
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|-
|-
| SUIT-009_AmR_mt_D021
| SUIT-009_AmR_mt_D021
| D021
| 1S
| 2D
| 3P
| 4Rot
| 5Ama
|  
|  
|  
|  
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| 5
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|-
|-
| SUIT-009_O2_ce-pce_D016
| SUIT-009_O2_ce-pce_D016
| D016
| ce1
| 1Dig
| 1S
| 2D
| 3P
| 4Rot
| 5Ama
|  
|  
|  
|  
Line 979: Line 964:
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| 7
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|-
|-
| SUIT-009_O2_mt_D015
| SUIT-009_O2_mt_D015
| D015
| 1S
| 2D
| 3P
| 4Rot
| 5Ama
|  
|  
|  
|  
Line 1,005: Line 988:
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| 5
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|-
|-
| SUIT-010_O2_ce-pce_D008
| SUIT-010_O2_ce-pce_D008
| D008
| ce1
| ce2Rot
| ce3S
| ce4D
| 1Dig
| 1c
|  
|  
|  
|  
Line 1,028: Line 1,012:
|  
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|  
|  
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| 6
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| Digitonin test
| A: Optimization of digitonin concentration for pce
|-
|-
| SUIT-011_O2_pfi_D024
| SUIT-011_O2_pfi_D024
| D024
| 1GM
| 2D
| 2c
| 3S
| 4U
| 5Rot
| 6Ama
|  
|  
|  
|  
Line 1,051: Line 1,036:
|  
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|  
|  
|  
| 7
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| GM+S_OXPHOS+Rot_ET
| A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control
|-
|-
| SUIT-012_O2_ce-pce_D052
| SUIT-012_O2_ce-pce_D052
| D052
| ce1
| 1Dig
| 1PM
| 2D
| 2c
| 3G
| 4U
| 5Ama
|  
|  
|  
|  
Line 1,074: Line 1,060:
|  
|  
|  
|  
|  
| 8
|
|
|
|
|
|
|
| PM+G_OXPHOS
| A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
|-
|-
| SUIT-012_O2_mt_D027
| SUIT-012_O2_mt_D027
| D027
| 1PM
| 2D
| 2c
| 3G
| 4U
| 5Ama
|  
|  
|  
|  
Line 1,100: Line 1,084:
|  
|  
|  
|  
|  
| 6
|
|
|
|
|
| PM+G_OXPHOS
| A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
|-
|-
| SUIT-013_AmR_ce_D023
| SUIT-013_AmR_ce_D023
| D023
| ce1
|  
|  
|  
|  
Line 1,129: Line 1,108:
|  
|  
|  
|  
|  
| 1
|
|
|-
|-
| SUIT-014_O2_pfi_D042
| SUIT-014_O2_pfi_D042
| D042
| 1GM
| 2D
| 3P
| 4S
| 5U
| 6Rot
| 7Ama
|  
|  
|  
|  
Line 1,147: Line 1,132:
|  
|  
|  
|  
|  
| 7
|
|
|
|
|
|
| GM+P+S_OXPHOS+Rot_ET
| A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
|-
|-
| SUIT-015_O2_pti_D043
| SUIT-015_O2_pti_D043
| D043
| 1OctM
| 2D
| 3G
| 4P
| 5S
| 6U
| 7Rot
| 8Ama
|  
|  
|  
|  
Line 1,170: Line 1,156:
|  
|  
|  
|  
|  
| 8
|
|
|
|
|
|
|
| F+G+P+S_OXPHOS+Rot_ET
| A:  F-pathway in LEAK state and OXPHOS state
|-
|-
| SUIT-016_O2_pfi_D044
| SUIT-016_O2_pfi_D044
| D044
| 1OctM
| 2D
| 3G
| 4S
| 5Rot
| 6Omy
| 7U
| 8Ama
|  
|  
|  
|  
Line 1,194: Line 1,180:
|  
|  
|  
|  
|  
| 8
|
|
|
|
|
|
|
| F+G+S+Rot_OXPHOS+Omy
| A: F-pathway in LEAK state and OXPHOS state
|-
|-
| SUIT-017_O2_mt _D046
| SUIT-017_O2_mt _D046
| D046
| 1OctM
| 2D
| 3G
| 3c
| 4S
| 5U
| 6Rot
| 7Ama
|  
|  
|  
|  
Line 1,218: Line 1,204:
|  
|  
|  
|  
|  
| 8
|
|
|
|
|
|
|
| F+G+S_OXPHOS+Rot_ET
| A:
|-
|-
| SUIT-017_O2_pfi_D049
| SUIT-017_O2_pfi_D049
| D049
| 1OctM
| 2D
| 2c
| 3G
| 4S
| 5U
| 6Rot
| 7Ama
|  
|  
|  
|  
Line 1,242: Line 1,228:
|  
|  
|  
|  
|  
| 8
|
|
|
|
|
|
|
| F+G+S_OXPHOS+Rot_ET
| A:
|-
|-
| SUIT-018_AmR_mt_D031
| SUIT-018_AmR_mt_D031
| D031
| 1GMS
| 2D
| 3Ama
|  
|  
|  
|  
Line 1,271: Line 1,252:
|  
|  
|  
|  
|  
| 3
|
|
|
|
|-
|-
| SUIT-018_AmR_mt_D040
| SUIT-018_AmR_mt_D040
| D040
| 1GMS
| 2D
| 3Ama
|  
|  
|  
|  
Line 1,295: Line 1,276:
|  
|  
|  
|  
|  
| 3
|
|
|
|
|-
|-
| SUIT-018_AmR_mt_D041
| SUIT-018_AmR_mt_D041
| D041
| 1GMS
| 2D
| 3Ama
|  
|  
|  
|  
Line 1,319: Line 1,300:
|  
|  
|  
|  
|  
| 3
|
|
|
|
|-
|-
| SUIT-018_O2_mt_D054
| SUIT-018_O2_mt_D054
| D054
| 1GMS
| 2D
| 3Ama
|  
|  
|  
|  
Line 1,343: Line 1,324:
|  
|  
|  
|  
|  
| 3
|
|
|
|
|-
|-
| SUIT-019_O2_pfi_D045
| SUIT-019_O2_pfi_D045
| D045
| 1PalM
| 2D
| 2c
| 03-Oct
| 4P
| 5G
| 6U
| 7S
| 8Rot
| 9Ama
|  
|  
|  
|  
Line 1,360: Line 1,348:
|  
|  
|  
|  
|  
| 10
|
|
|
|
|
|
|
|
|
| Pal+Oct+P+G_OXPHOS+S+Rot_ET
| A:
|-
|-
| SUIT-020_Fluo_mt_D033
| SUIT-020_Fluo_mt_D033
| D033
| 1PM
| 2D
| 3G
| 4S
| 5Rot
| 6Omy
| 7U
| 8Ama
|  
|  
|  
|  
Line 1,386: Line 1,372:
|  
|  
|  
|  
| 8
|-
| SUIT-020_O2_mt_D032
| D032
| 1PM
| 2D
| 2c
| 3G
| 4S
| 5Rot
| 6Omy
| 7U
| 8Ama
|  
|  
|  
|  
Line 1,393: Line 1,392:
|  
|  
|  
|  
|
| PM+G+S+Rot_OXPHOS+Omy
| A: simultaneous determination of O2 flux and mt-membrane potential
|-
| SUIT-020_Fluo_mt_D033_chemical_background
|  
|  
|  
|  
|  
|  
|  
|  
| 9
|-
| SUIT-021_Fluo_mt_D036
| D036
| 1GM
| 2D
| 3S
| 4Rot
| 5Omy
| 6U
| 7Ama
|  
|  
|  
|  
Line 1,415: Line 1,420:
|  
|  
|  
|  
|  
| 7
|
|
| PM+G+S+Rot_OXPHOS+Omy
| A: simultaneous determination of O2 flux and mt-membrane potential
|-
|-
| SUIT-020_O2_mt_D032
| SUIT-021_O2_mt_D035
| D035
| 1GM
| 2D
| 2c
| 3S
| 4Rot
| 5Omy
| 6U
| 7Ama
|  
|  
|  
|  
Line 1,434: Line 1,444:
|  
|  
|  
|  
|  
| 8
|
|
|
|
|
|
|
| PM+G+S+Rot_OXPHOS+Omy
| A: simultaneous determination of O2 flux and mt-membrane potential
|-
|-
| SUIT-021_Fluo_mt_D036
| SUIT-022 O2 ce D051
| D051
| ce1
| ce2KCN
| ce3SHAM
|  
|  
|  
|  
Line 1,463: Line 1,468:
|  
|  
|  
|  
|  
| 3
|
|
| OXPHOS (GM+S+Rot+Omy)
| A: simultaneous determination of O2 flux and mt-membrane potential
|-
|-
| SUIT-021_Fluo_mt_D036_chemical background
| SUIT-023 O2 ce D053
|  
| D053
|  
| ce1
|  
| ce2SHAM
| ce3KCN
|  
|  
|  
|  
Line 1,490: Line 1,492:
|  
|  
|  
|  
| OXPHOS (GM+S+Rot+Omy)
| 3
| A: simultaneous determination of O2 flux and mt-membrane potential
|-
|-
| SUIT-021_O2_mt_D035
| SUIT-024_O2_ce-pce_D056
| D056
| ce1
| 1Dig
| 1PM
| 2T
| 2D
| 3Omy
| 4Ama
|  
|  
|  
|  
Line 1,507: Line 1,516:
|  
|  
|  
|  
|  
| 7
|
|
|
|
|
|
| OXPHOS (GM+S+Rot+Omy)
| A: simultaneous determination of O2 flux and mt-membrane potential
|-
|-
| SUIT-022 O2 ce D051
| SUIT-025_O2_mt_D057
| D057
| 1D
| 2M
| 03-Oct
| 3c
| 4M
| 5P
| 6G
| 7S
| 8Rot
| 9Ama
|  
|  
|  
|  
Line 1,528: Line 1,540:
|  
|  
|  
|  
|  
| 10
|
|
|
|
|
|
|
|
|
| AOX (ce CN+SHAM)
| A: Determination of the respiration due to the alternative oxidase pathway
|-
|-
| SUIT-023 O2 ce D053
| SUIT-026_AmR_mt_D064
| D064
| 1S
| 2Rot
| 3D
| 4Ama
|  
|  
|  
|  
Line 1,558: Line 1,564:
|  
|  
|  
|  
|  
| 4
|
|
|
| AOX-ce SHAM+CN
| A: Determination of respiration through the CIII-CIV pathway
|-
|-
| SUIT-024_O2_ce-pce_D056
| SUIT-026_O2_mt_D063
|  
| D063
|  
| 1S
| 2Rot
| 3D
| 3c
| 4Ama
|  
|  
|  
|  
Line 1,583: Line 1,588:
|  
|  
|  
|  
|  
| 5
|
|
| ATPase (PM)
| A: Determination of the presence of ATPases in mitochondrial preparations.
|-
|-
| SUIT-025_O2_mt_D057
| SUIT-027_O2_ce-pce_D065
|  
| D065
|  
| ce1
|  
| 1Dig
|  
| 1M
|  
| 2D
|  
| 3M
|  
| 4P
|  
| 5G
|  
|  
|  
|  
Line 1,610: Line 1,611:
|  
|  
|  
|  
| OXPHOS (F+M+P+G+S+Rot)
|  
|  
| 7
|-
|-
| SUIT-026_AmR_mt_D064
| SUIT-006_Fluo_mt_D034_chemical background
|
| D034_chemical backgr.
|  
|  
|  
|  
|  
Line 1,636: Line 1,636:
|  
|  
|  
|  
| 0
|-
|-
| SUIT-026_O2_mt_D063
| SUIT-020_Fluo_mt_D033_chemical_background
|
| D033_chemical backgr.
|  
|  
|  
|  
|  
Line 1,660: Line 1,660:
|  
|  
|  
|  
| 0
|-
|-
| SUIT-027_O2_ce-pce_D065
| SUIT-021_Fluo_mt_D036_chemical background
| D036_chemical backgr.
|  
|  
|  
|  
Line 1,682: Line 1,684:
|  
|  
|  
|  
| Malate anaplerosis
| 0
| A: Malate anaplerotic pathway
|}
|}

Revision as of 16:27, 8 July 2019

Attention! Attention!

Disclaimer: This is an alpha version and page is a currently still under development. Please DO NOT consider information below in this page to be true or of any scientific value.

This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.

Protocol name D number Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Step 12 Step 13 Step 14 Step 15 Step 16 Step 17 Step 18 Step 19 Step 20 Number of steps
SUIT-001_O2_ce-pce_D003 D003 ce1 1Dig 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 14
SUIT-001_O2_PBMC-PLT_D004 D004 ce1 1Dig 1PM 2D 2c 3U 4G 5S (6Oct) 7Rot 8Gp 9Ama 10AsTm 11Azd 14
SUIT-001_O2_mt_D001 D001 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 12
SUIT-001_O2_pfi_D002 D002 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 12
SUIT-002_O2_ce-pce_D007 D007 ce1 1Dig 1D 2M 4Oct 3c 4M 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16
SUIT-002_O2_ce-pce-PBMC-PLT_D007a D007a ce1 1Dig 1D 2M 4Oct 3c 4M 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16
SUIT-002_O2_mt_D005 D005 1D 2M 3Oct 3c 4M 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 14
SUIT-002_O2_pfi_D006 D006 1D 2M 3Oct 3c 4M 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 14
SUIT-003_AmR_ce_D017 D017 ce1 ce2P ce3Omy ce4U ce5Rot ce6S ce7Ama 7
SUIT-003_AmR_ce_D058 D058 ce1 ce1SOD ce1HRP ce1AmR ce2Omy ce3U ce4Rot ce5Ama 8
SUIT-003_AmR_ce_D059 D059 ce1 ce1H2O ce1MiR05 ce1DMSO ce2Omy ce3U ce4Rot ce5Ama 8
SUIT-003_O2_ce_D009 D009 ce1 ce2Omy ce3U ce4Rot ce5Ama 5
SUIT-003_O2_ce_D012 D012 ce1 ce1P ce2Omy ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce_D028 D028 ce1 ce2Omy ce3U ce4Rot ce5S ce6Ama 6
SUIT-003_O2_ce_D037 D037 ce1 ce1Glc (ce2Omy) ce3U ce4Ama 5
SUIT-003_O2_ce_D038 D038 ce1 (ce2Omy) ce3U ce3Glc ce3'U ce4Ama 6
SUIT-003_O2_ce_D039 D039 ce1 (ce2Omy) ce3U ce4Rot ce5Ama 5
SUIT-003_O2_ce_D050 D050 ce1 ce1Snv (ce2Omy) ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce_D060 D060 ce1 (ce2Omy) ce3U ce4Rot ce5Snv ce6Mnanv ce7Ama 7
SUIT-003_O2_ce_D061 D061 ce1 (ce2Omy) ce3U ce4Rot ce5DMSO ce6DMSO ce7Ama 7
SUIT-003_O2_ce_D062 D062 ce1 ce1DMSO (ce2Omy) ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce-pce_D013 D013 ce1 ce1P ce2Omy ce3U ce3Glc ce3M ce4Rot ce5S 1Dig 1U 1c 2Ama 3AsTm 4Azd 14
SUIT-003_O2_ce-pce_D018 D018 ce1 ce1P ce2Omy ce3U ce3Glc ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 12
SUIT-003_O2_ce-pce_D020 D020 ce1 ce1P ce2Omy ce3U ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 11
SUIT-004_O2_pfi_D010 D010 1PM 2D 2c 3U 4S 5Rot 6Ama 7AsTm 8Azd 9
SUIT-005_O2_pfi_D011 D011 1OctM 2D 3P 4S 5U 6Rot 7Ama 8AsTm 9Azd 9
SUIT-006_AmR_mt_D048 D048 1PM 2D 3Omy 4U 5Ama 5
SUIT-006_Fluo_mt_D034 D034 1PM 2D 3Omy 4U 5Ama 5
SUIT-006_MgG_mt_D055 D055 1PM 2D 3Cat 4U 5Ama 5
SUIT-006_O2_ce-pce_D029 D029 ce1 1Dig 1PM 2D 2c 3Omy 4U 5Ama 8
SUIT-006_O2_mt_D022 D022 1SRot 2D 2c (3Omy) 4U 5Ama 6
SUIT-006_O2_mt_D047 D047 1PM 2D 2c 3Omy 4U 5Ama 6
SUIT-007_O2_ce-pce_D030 D030 ce1 1Dig 1G 2D 2c 3M 4U 5Ama 8
SUIT-008_O2_ce-pce_D025 D025 ce1 1Dig 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 12
SUIT-008_O2_mt_D026 D026 1PM 2D 2c 3G 4S 4D 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-008_O2_pfi_D014 D014 1PM 2D 2c 3G 4S 4D 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-009_AmR_ce-pce_D019 D019 ce1 1Dig 1S 2D 3P 4Rot 5Ama 7
SUIT-009_AmR_mt_D021 D021 1S 2D 3P 4Rot 5Ama 5
SUIT-009_O2_ce-pce_D016 D016 ce1 1Dig 1S 2D 3P 4Rot 5Ama 7
SUIT-009_O2_mt_D015 D015 1S 2D 3P 4Rot 5Ama 5
SUIT-010_O2_ce-pce_D008 D008 ce1 ce2Rot ce3S ce4D 1Dig 1c 6
SUIT-011_O2_pfi_D024 D024 1GM 2D 2c 3S 4U 5Rot 6Ama 7
SUIT-012_O2_ce-pce_D052 D052 ce1 1Dig 1PM 2D 2c 3G 4U 5Ama 8
SUIT-012_O2_mt_D027 D027 1PM 2D 2c 3G 4U 5Ama 6
SUIT-013_AmR_ce_D023 D023 ce1 1
SUIT-014_O2_pfi_D042 D042 1GM 2D 3P 4S 5U 6Rot 7Ama 7
SUIT-015_O2_pti_D043 D043 1OctM 2D 3G 4P 5S 6U 7Rot 8Ama 8
SUIT-016_O2_pfi_D044 D044 1OctM 2D 3G 4S 5Rot 6Omy 7U 8Ama 8
SUIT-017_O2_mt _D046 D046 1OctM 2D 3G 3c 4S 5U 6Rot 7Ama 8
SUIT-017_O2_pfi_D049 D049 1OctM 2D 2c 3G 4S 5U 6Rot 7Ama 8
SUIT-018_AmR_mt_D031 D031 1GMS 2D 3Ama 3
SUIT-018_AmR_mt_D040 D040 1GMS 2D 3Ama 3
SUIT-018_AmR_mt_D041 D041 1GMS 2D 3Ama 3
SUIT-018_O2_mt_D054 D054 1GMS 2D 3Ama 3
SUIT-019_O2_pfi_D045 D045 1PalM 2D 2c 03-Oct 4P 5G 6U 7S 8Rot 9Ama 10
SUIT-020_Fluo_mt_D033 D033 1PM 2D 3G 4S 5Rot 6Omy 7U 8Ama 8
SUIT-020_O2_mt_D032 D032 1PM 2D 2c 3G 4S 5Rot 6Omy 7U 8Ama 9
SUIT-021_Fluo_mt_D036 D036 1GM 2D 3S 4Rot 5Omy 6U 7Ama 7
SUIT-021_O2_mt_D035 D035 1GM 2D 2c 3S 4Rot 5Omy 6U 7Ama 8
SUIT-022 O2 ce D051 D051 ce1 ce2KCN ce3SHAM 3
SUIT-023 O2 ce D053 D053 ce1 ce2SHAM ce3KCN 3
SUIT-024_O2_ce-pce_D056 D056 ce1 1Dig 1PM 2T 2D 3Omy 4Ama 7
SUIT-025_O2_mt_D057 D057 1D 2M 03-Oct 3c 4M 5P 6G 7S 8Rot 9Ama 10
SUIT-026_AmR_mt_D064 D064 1S 2Rot 3D 4Ama 4
SUIT-026_O2_mt_D063 D063 1S 2Rot 3D 3c 4Ama 5
SUIT-027_O2_ce-pce_D065 D065 ce1 1Dig 1M 2D 3M 4P 5G 7
SUIT-006_Fluo_mt_D034_chemical background D034_chemical backgr. 0
SUIT-020_Fluo_mt_D033_chemical_background D033_chemical backgr. 0
SUIT-021_Fluo_mt_D036_chemical background D036_chemical backgr. 0
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