Dube 2014 Am J Physiol Endocrinol Metab

[[Has title::Dube JJ, Coen PM, DiStefano G, Chacon AC, Helbling NL, Desimone ME, Stafanovic-Racic M, Hames KC, Despines AA, Toledo FG, Goodpaster BH (2014) Effects of acute lipid overload on skeletal muscle insulin resistance, metabolic flexibility, and mitochondrial performance. Am J Physiol Endocrinol Metab 307:E1117-24.]]

» [[Has info::PMID: 25352435 Open Access]]

Was written by::Dube JJ, Was written by::Coen PM, Was written by::DiStefano G, Was written by::Chacon AC, Was written by::Helbling NL, Was written by::Desimone ME, Was written by::Stafanovic-Racic M, Was written by::Hames KC, Was written by::Despines AA, Was written by::Toledo FG, Was written by::Goodpaster BH (Was published in year::2014) Was published in journal::Am J Physiol Endocrinol Metab

Abstract: [[has abstract::We hypothesized that acute lipid-induced insulin resistance would be attenuated in high-oxidative muscle of lean trained (LT) endurance athletes due to their enhanced metabolic flexibility and mitochondrial capacity. Lean sedentary (LS), obese sedentary (OS), and LT participants completed two hyperinsulinemic euglycemic clamp studies with and without (glycerol control) the coinfusion of Intralipid. Metabolic flexibility was measured by indirect calorimetry as the oxidation of fatty acids and glucose during fasted and insulin-stimulated conditions, the latter with and without lipid oversupply. Muscle biopsies were obtained for mitochondrial and insulin-signaling studies. During hyperinsulinemia without lipid, glucose infusion rate (GIR) was lowest in OS due to lower rates of nonoxidative glucose disposal (NOGD), whereas state 4 respiration was increased in all groups. Lipid infusion reduced GIR similarly in all subjects and reduced state 4 respiration. However, in LT subjects, fat oxidation was higher with lipid oversupply, and although glucose oxidation was reduced, NOGD was better preserved compared with LS and OS subjects. Mitochondrial performance was positively associated with better NOGD and insulin sensitivity in both conditions. We conclude that enhanced mitochondrial performance with exercise is related to better metabolic flexibility and insulin sensitivity in response to lipid overload.]] • Keywords: has publicationkeywords::Lipids, has publicationkeywords::Mitochondria; Skeletal Muscle

• O2k-Network Lab: Was published by MiPNetLab::US FL Orlando Goodpaster BH

Labels: MiParea: MiP area::Respiration, MiP area::Exercise physiology;nutrition;life style  Pathology: Diseases::Diabetes 

Organism: Organism::Human  Tissue;cell: tissue and cell::Skeletal muscle  Preparation: Preparation::Permeabilized tissue 

Coupling state: Coupling states::LEAK, Coupling states::OXPHOS, Coupling states::ET 

HRR: Instrument and method::Oxygraph-2k 

additional label::BMI, additional label::VO2max