Dos Santos 2013 Bio Open
» [[Has info::doi: 10.1242/bio.20122295 Open Access]]
Was written by::Dos Santos RS, Was written by::Galina A, Was written by::Da-Silva WS (Was published in year::2013) Was published in journal::Bio Open
Abstract: [[has abstract::Goldfish have been used for cold acclimation studies, which have focused on changes in glycolytic and oxidative enzymes or alterations in lipid composition in skeletal muscle. Here we examine the effects of cold acclimation on the functional properties of isolated mitochondria and permeabilized fibers from goldfish white skeletal muscle, focusing on understanding the types of changes that occur in the mitochondrial respiratory states. We observed that cold acclimation promoted a significant increase in the mitochondrial oxygen consumption rates. Western blot analysis showed that UCP3 was raised by ∼1.5-fold in cold-acclimated muscle mitochondria. Similarly, we also evidenced a rise in the adenine nucleotide translocase content in cold-acclimated muscle mitochondria compared to warm-acclimated mitochondria (0.96±0.05 vs 0.68±0.02 nmol carboxyatractyloside mg−1 protein). This was followed by a 2-fold increment in the citrate synthase activity, which suggests a higher mitochondrial content in cold-acclimated goldfish. Even with higher levels of UCP3 and ANT, the effects of activator (palmitate) and inhibitors (carboxyatractyloside and GDP) on mitochondrial parameters were similar in both warm- and cold-acclimated goldfish. Thus, we propose that cold acclimation in goldfish promotes an increase in functional oxidative capacity, with higher mitochondrial content without changes in the mitochondrial uncoupling pathways.]] • Keywords: has publicationkeywords::Cold acclimation, has publicationkeywords::Mitochondria, has publicationkeywords::Skeletal muscle, has publicationkeywords::Goldfish
• O2k-Network Lab: Was published by MiPNetLab::BR Rio de Janeiro Galina A
Labels:
Organism: Organism::Fishes
Tissue;cell: tissue and cell::Skeletal muscle
Preparation: Preparation::Permeabilized tissue, Preparation::Isolated mitochondria
Regulation: Topic::Temperature Coupling state: Coupling states::LEAK, Coupling states::OXPHOS, Coupling states::ETS Pathway: Pathways::N, Pathways::S HRR: Instrument and method::Oxygraph-2k
