Dohlmann 2014 Abstract MiP2014: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=Mitochondrial adaptations to high-intensity training in young and elderly men and women. | |title=Mitochondrial adaptations to high-intensity training in young and elderly men and women. Mitochondr Physiol Network 19.13. | ||
|info=[[File:Dohlmann_T.jpg|150px|right|Dohlmann T]] [http://www.mitophysiology.org/index.php?mip2014 MiP2014], [[Laner 2014 Mitochondr Physiol Network MiP2014|Book of Abstracts Open Access]] | |info=[[File:Dohlmann_T.jpg|150px|right|Dohlmann T]] [http://www.mitophysiology.org/index.php?mip2014 MiP2014], [[Laner 2014 Mitochondr Physiol Network MiP2014|Book of Abstracts Open Access]] | ||
|authors=Dohlmann T, Dela F, Helge JW, Larsen S | |authors=Dohlmann T, Dela F, Helge JW, Larsen S |
Revision as of 10:32, 12 August 2014
Mitochondrial adaptations to high-intensity training in young and elderly men and women. Mitochondr Physiol Network 19.13. |
Link:
MiP2014, Book of Abstracts Open Access
Dohlmann T, Dela F, Helge JW, Larsen S (2014)
Event: MiP2014
This study investigated how skeletal muscle mitochondria adapt to high intensity interval training (HIT) and whether adaptations differed according to age.
Two groups of healthy sedentary adults completed 18 sessions of low volume HIT (5x1 min @ ~132% of Wmax with 1.5 min rest periods). The groups were matched for BMI and baseline VO2max but differed in age (P<0.001). One group (N=9) aged 36Β±3 years (BMI 34Β±2 kgβ’m-2 and VO2max 2.7Β±0.2 lβ’min-1) was considered young (YOUNG), and the other group (N=4) aged 67Β±2 years (BMI 32Β±1 kgβ’m-2 and VO2max 2.1Β±0.3 lβ’min-1) was considered old (OLD). Mitochondrial respiration was measured using high-resolution respirometry (OROBOROS Oxygraph-2k) in permeabilized muscle fibers from the vastus lateralis, and mitochondrial ADP sensitivity was determined using Michaelis-Menten kinetics.
Following training, YOUNG increased VO2max by 7% (P=0.003), with no change in OLD. Mitochondrial capacity for oxidative phosphorylation (OXPHOS) was increased in both groups (48Β±2 to 84Β±4 pmol O2β’s-1β’mg-1 in YOUNG P<0.001 and 59Β±9 to 71Β±11 pmol O2β’s-1β’mg-1 in OLD P<0.05), and maximal noncoupled respiration was increased in YOUNG (59Β±4 to 92Β±5 pmol O2β’s-1β’mg-1, P<0.05) but not in OLD (71Β±9 to 80Β±10 pmol O2β’sβ1β’mgβ1). Mitochondrial ADP sensitivity did not change following training in either group (Kmapp [ADP] 0.19Β±0.10 to 0.16Β±0.04 in YOUNG, and 0.26Β±0.18 to 0.15Β±0.06 in OLD), but maximal ADP stimulated respiration (Jmax) increased in YOUNG (11Β±1 to 21Β±2 pmol O2β’s-1β’mg-1, P<0.05) but not in OLD (13Β±2 to 17Β±2 pmol O2β’s-1β’mg-1).
Our results indicate that the mitochondrial adaptation to HIT in elderly men and women move in the same direction as younger subjects, although only OXPHOS capacity increased significantly in OLD. Due to a low number of subjects in OLD, inclusion of additional subjects is necessary to elucidate exactly how elderly men and women adapt to HIT, compared with younger controls.
β’ O2k-Network Lab: DK Copenhagen Dela F
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style Pathology: Aging; senescence"Aging; senescence" is not in the list (Aging;senescence, Alzheimer's, Autism, Cancer, Cardiovascular, COPD, Diabetes, Inherited, Infectious, Myopathy, ...) of allowed values for the "Diseases" property.
Organism: Human Tissue;cell: Skeletal muscle Preparation: Permeabilized cells
Regulation: ADP Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
MiP2014:HIT
Affiliation
Xlab, Center Healthy Aging, Dep Biomed Sc, Fac Health Sc, Univ Copenhagen, Denmark. β [email protected]