Dithionite

high-resolution terminology - matching measurements at high-resolution

Dithionite

Description

Zero oxygen solution powder, Na₂S₂O₄, used for calibration of oxygen sensors at zero oxygen, or for stepwise reduction of oxygen concentration in instrumental O₂ background tests.

Abbreviation: Dit

Reference: MiPNet06.03 POS-calibration-SOP, MiPNet14.06 Instrumental O2 background

MitoPedia methods: Respirometry 

Preparation

Preparation of 30 mM dithionite solution for instrumental oxygen background (dissolved in Phosphate buffer, 50 mM, pH 8)

1. Weigh 0.051 g dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
2. Adjust final volume to 10 mL with Phosphate buffer and keep closed to avoid decomposing.

Preparation of 10 mM dithionite solution for instrumental oxygen background (dissolved in Phosphate buffer, 50 mM, pH 8)

1. Weigh 0.017 g dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
2. Adjust final volume to 10 mL with Phosphate buffer and keep closed to avoid decomposing.

Note: Experiments performed with 10 and 30 mM dithionite solution showed identical results. However, sodium dithionite is slowly oxidized by air. When dithionite is open for long time, we recommend to use a concentration of 30 mM for performing the instrumental oxygen background. For new dithionite, 10 mM dithionite solution may be used.

• From MiPNet06.03 POS-calibration-SOP (Version 14): Prepare "zero solution" by dissolving c. 20 mg sodium hydrosulfite (OroboPOS-Service Kit) or two tips of a spatula in 0.5 ml of water. Mix in a small vial with minimum gas space. Use fresh. Dithionite may not work after prolonged storage.

Further information »Talk:Dithionite