Difference between revisions of "Dithionite"

Revision as of 16:21, 2 October 2018

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Dithionite

Description

Zero oxygen solution powder, Na₂S₂O₄, used for calibration of oxygen sensors at zero oxygen, or for stepwise reduction of oxygen concentration in instrumental O₂ background tests.

Abbreviation: Dit

Reference: MiPNet06.03 POS-calibration-SOP, MiPNet14.06 Instrumental O2 background

MitoPedia methods: Respirometry

Preparation

Preparation of 30 mM dithionite solution for instrumental oxygen background (dissolved in Phosphate buffer, 50 mM, pH 8)

Weigh 0.051 g dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
Adjust final volume to 10 mL with Phosphate buffer and keep closed to avoid decomposing.

Preparation of 10 mM dithionite solution for instrumental oxygen background (dissolved in Phosphate buffer, 50 mM, pH 8)

Weigh 0.017 g dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
Adjust final volume to 10 mL with Phosphate buffer and keep closed to avoid decomposing.

Note: Experiments performed with 10 and 30 mM dithionite solution showed identical results. However, sodium dithionite is slowly oxidized by air. When dithionite is open for long time, we recommend to use a concentration of 30 mM for performing the instrumental oxygen background. For new dithionite, 10 mM dithionite solution may be used.

From MiPNet06.03 POS-calibration-SOP (Version 14): Prepare "zero solution" by dissolving c. 20 mg sodium hydrosulfite (OroboPOS-Service Kit) or two tips of a spatula in 0.5 ml of water. Mix in a small vial with minimum gas space. Use fresh. Dithionite may not work after prolonged storage.

Further information »Talk:Dithionite

@@ Line 11: / Line 11: @@
 {{MitoPedia topics}}
 == Preparation ==
-::: '''Preparation of 10 mM Dithionite solution for Instrumental Background''' (dissolved in Phosphate buffer, 50 mM, pH 8)
+::: '''Preparation of 30 mM dithionite solution for instrumental oxygen background''' (dissolved in Phosphate buffer, 50 mM, pH 8)
-::::# Weigh 0.017 g Dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
+::::# Weigh 0.051 g dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
-::::# Adjust final volume to 10 ml with Phosphate buffer and keep closed to avoid decomposing.
+::::# Adjust final volume to 10 mL with Phosphate buffer and keep closed to avoid decomposing.
-:::: Note: Sodium dithionite cannot be stored for a long period of time, because it is slowly oxidized by air when in solution. Even with the absence of air, solutions of sodium dithionite deteriorate. Therefore when Dithionite is open for a long time, we use a concentration of 30 mM for the Instrumental Background. The experiments showed the identical results with 10 and 30 mM Dithionite stock.
+::: '''Preparation of 10 mM dithionite solution for instrumental oxygen background''' (dissolved in Phosphate buffer, 50 mM, pH 8)
+::::# Weigh 0.017 g dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
+::::# Adjust final volume to 10 mL with Phosphate buffer and keep closed to avoid decomposing.
+:::: Note: Experiments performed with 10 and 30 mM dithionite solution showed identical results. However, sodium dithionite is slowly oxidized by air. When dithionite is open for long time, we recommend to use a concentration of 30 mM for performing the instrumental oxygen background. For new dithionite, 10 mM dithionite solution may be used.