Difference between revisions of "Dithionite"
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|abbr=Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub> | |abbr=Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub> or Dit | ||
|description=Zero oxygen solution powder, Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub>, used for [[MiPNet19.18D O2k-calibration |calibration of oxygen sensors]] at zero oxygen, or for stepwise reduction of oxygen concentration in [[MiPNet14.06 Instrumental O2 background |instrumental O<sub>2</sub> background tests]]. | |description=Zero oxygen solution powder, Na<sub>2</sub>S<sub>2</sub>O<sub>4</sub>, used for [[MiPNet19.18D O2k-calibration |calibration of oxygen sensors]] at zero oxygen, or for stepwise reduction of oxygen concentration in [[MiPNet14.06 Instrumental O2 background |instrumental O<sub>2</sub> background tests]]. | ||
|info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet14.06 Instrumental O2 background]] | |info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet14.06 Instrumental O2 background]] | ||
}} | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
}} | }} | ||
{{MitoPedia O2k and high-resolution respirometry}} | |||
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== Preparation == | == Preparation == | ||
::: '''Preparation of 10 mM Dithionite solution for Instrumental Background''' (dissolved in Phosphate buffer, 50 mM, pH 8) | ::: '''Preparation of 10 mM Dithionite solution for Instrumental Background''' (dissolved in Phosphate buffer, 50 mM, pH 8) | ||
Revision as of 12:54, 22 August 2017
- high-resolution terminology - matching measurements at high-resolution
Dithionite
Description
Zero oxygen solution powder, Na2S2O4, used for calibration of oxygen sensors at zero oxygen, or for stepwise reduction of oxygen concentration in instrumental O2 background tests.
Abbreviation: Na2S2O4 or Dit
Reference: MiPNet06.03 POS-calibration-SOP, MiPNet14.06 Instrumental O2 background
MitoPedia methods: Respirometry
Preparation
- Preparation of 10 mM Dithionite solution for Instrumental Background (dissolved in Phosphate buffer, 50 mM, pH 8)
- Weigh 0.017 g Dithionite, sodium hydrosulfite, and transfer to 10 mL volumetric glass flask.
- Adjust final volume to 10 ml with Phosphate buffer and keep closed to avoid decomposing.
- Note: Sodium dithionite cannot be stored for a long period of time, because it is slowly oxidized by air when in solution. Even with the absence of air, solutions of sodium dithionite deteriorate. Therefore when Dithionite is open for a long time, we use a concentration of 30 mM for the Instrumental Background. The experiments showed the identical results with 10 and 30 mM Dithionite stock.
- From MiPNet06.03 POS-calibration-SOP (Version 14): Prepare "zero solution" by dissolving c. 20 mg sodium hydrosulfite (OroboPOS-Service Kit) or two tips of a spatula in 0.5 ml of water. Mix in a small vial with minimum gas space. Use fresh. Dithionite may not work after prolonged storage.
Further information »Talk:Dithionite
