Difference between revisions of "De Paula Nascimento-Castro 2022 Biomedicines"
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|title=de Paula Nascimento-Castro C, Winkelmann-Duarte EC, Mancini G, Welter PG, Plácido E, Farina M, Gil-Mohapel J, Rodrigues ALS, de Bem AF, Brocardo PS (2022) Temporal characterization of behavioral and hippocampal dysfunction in the YAC128 mouse model of Huntington's disease. https://doi.org/10.3390/biomedicines10061433 | |title=de Paula Nascimento-Castro C, Winkelmann-Duarte EC, Mancini G, Welter PG, Plácido E, Farina M, Gil-Mohapel J, Rodrigues ALS, de Bem AF, Brocardo PS (2022) Temporal characterization of behavioral and hippocampal dysfunction in the YAC128 mouse model of Huntington's disease. https://doi.org/10.3390/biomedicines10061433 | ||
|info=Biomedicines 10:1433. [https://pubmed.ncbi.nlm.nih.gov/35740454 PMID: 35740454 Open Access] | |info=Biomedicines 10:1433. [https://pubmed.ncbi.nlm.nih.gov/35740454 PMID: 35740454 Open Access] | ||
|authors=de Paula Nascimento-Castro | |authors=de Paula Nascimento-Castro Cristine, Winkelmann-Duarte Elisa C, Mancini Gianni, Gomes Welter Priscilla, Placido Evelini, Farina Marcelo, Gil-Mohapel Joana, Rodrigues Ana Lucia S, De Bem Andreza Fabro, Brocardo Patricia S | ||
|year=2022 | |year=2022 | ||
|journal=Biomedicines | |journal=Biomedicines | ||
|abstract=Huntington's disease (HD) is a genetic neurodegenerative disease characterized by motor, psychiatric, and cognitive symptoms. Emerging evidence suggests that emotional and cognitive deficits seen in HD may be related to hippocampal dysfunction. We used the YAC128 HD mouse model to perform a temporal characterization of the behavioral and hippocampal dysfunctions. Early and late symptomatic YAC128 mice exhibited depressive-like behavior, as demonstrated by increased immobility times in the Tail Suspension Test. In addition, YAC128 mice exhibited cognitive deficits in the Swimming T-maze Test during the late symptomatic stage. Except for a reduction in basal mitochondrial respiration, no significant deficits in the mitochondrial respiratory rates were observed in the hippocampus of late symptomatic YAC128 mice. In agreement, YAC128 animals did not present robust alterations in mitochondrial ultrastructural morphology. However, light and electron microscopy analysis revealed the presence of dark neurons characterized by the intense staining of granule cell bodies and shrunken nuclei and cytoplasm in the hippocampal dentate gyrus (DG) of late symptomatic YAC128 mice. Furthermore, structural alterations in the rough endoplasmic reticulum and Golgi apparatus were detected in the hippocampal DG of YAC128 mice by electron microscopy. These results clearly show a degenerative process in the hippocampal DG in late symptomatic YAC128 animals. | |abstract=Huntington's disease (HD) is a genetic neurodegenerative disease characterized by motor, psychiatric, and cognitive symptoms. Emerging evidence suggests that emotional and cognitive deficits seen in HD may be related to hippocampal dysfunction. We used the YAC128 HD mouse model to perform a temporal characterization of the behavioral and hippocampal dysfunctions. Early and late symptomatic YAC128 mice exhibited depressive-like behavior, as demonstrated by increased immobility times in the Tail Suspension Test. In addition, YAC128 mice exhibited cognitive deficits in the Swimming T-maze Test during the late symptomatic stage. Except for a reduction in basal mitochondrial respiration, no significant deficits in the mitochondrial respiratory rates were observed in the hippocampus of late symptomatic YAC128 mice. In agreement, YAC128 animals did not present robust alterations in mitochondrial ultrastructural morphology. However, light and electron microscopy analysis revealed the presence of dark neurons characterized by the intense staining of granule cell bodies and shrunken nuclei and cytoplasm in the hippocampal dentate gyrus (DG) of late symptomatic YAC128 mice. Furthermore, structural alterations in the rough endoplasmic reticulum and Golgi apparatus were detected in the hippocampal DG of YAC128 mice by electron microscopy. These results clearly show a degenerative process in the hippocampal DG in late symptomatic YAC128 animals. | ||
|keywords=Huntington’s disease, YAC128 mice, Electron microscopy, Hippocampus, Neurodegeneration | |||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
|mipnetlab=BR Brasilia De Bem AF | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
|diseases=Neurodegenerative | |||
|organism=Mouse | |||
|tissues=Nervous system | |||
|preparations=Homogenate | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, S, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2022-11 | |additional=2022-11 | ||
}} | }} | ||
Latest revision as of 13:48, 22 November 2022
| de Paula Nascimento-Castro C, Winkelmann-Duarte EC, Mancini G, Welter PG, Plácido E, Farina M, Gil-Mohapel J, Rodrigues ALS, de Bem AF, Brocardo PS (2022) Temporal characterization of behavioral and hippocampal dysfunction in the YAC128 mouse model of Huntington's disease. https://doi.org/10.3390/biomedicines10061433 |
» Biomedicines 10:1433. PMID: 35740454 Open Access
de Paula Nascimento-Castro Cristine, Winkelmann-Duarte Elisa C, Mancini Gianni, Gomes Welter Priscilla, Placido Evelini, Farina Marcelo, Gil-Mohapel Joana, Rodrigues Ana Lucia S, De Bem Andreza Fabro, Brocardo Patricia S (2022) Biomedicines
Abstract: Huntington's disease (HD) is a genetic neurodegenerative disease characterized by motor, psychiatric, and cognitive symptoms. Emerging evidence suggests that emotional and cognitive deficits seen in HD may be related to hippocampal dysfunction. We used the YAC128 HD mouse model to perform a temporal characterization of the behavioral and hippocampal dysfunctions. Early and late symptomatic YAC128 mice exhibited depressive-like behavior, as demonstrated by increased immobility times in the Tail Suspension Test. In addition, YAC128 mice exhibited cognitive deficits in the Swimming T-maze Test during the late symptomatic stage. Except for a reduction in basal mitochondrial respiration, no significant deficits in the mitochondrial respiratory rates were observed in the hippocampus of late symptomatic YAC128 mice. In agreement, YAC128 animals did not present robust alterations in mitochondrial ultrastructural morphology. However, light and electron microscopy analysis revealed the presence of dark neurons characterized by the intense staining of granule cell bodies and shrunken nuclei and cytoplasm in the hippocampal dentate gyrus (DG) of late symptomatic YAC128 mice. Furthermore, structural alterations in the rough endoplasmic reticulum and Golgi apparatus were detected in the hippocampal DG of YAC128 mice by electron microscopy. These results clearly show a degenerative process in the hippocampal DG in late symptomatic YAC128 animals. • Keywords: Huntington’s disease, YAC128 mice, Electron microscopy, Hippocampus, Neurodegeneration • Bioblast editor: Plangger M • O2k-Network Lab: BR Brasilia De Bem AF
Labels: MiParea: Respiration
Pathology: Neurodegenerative
Organism: Mouse Tissue;cell: Nervous system Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
2022-11
