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DatLab

From Bioblast

                



DatLab

O2k-Catalogue

Description DatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for High-Resolution FluoRespirometry with the O2k-FluoRespirometer.

The newest DatLab version is included in the O2k-FluoRespirometer, and updates of DatLab are free of charge during the warranty period of the O2k.

Product ID 20700
Type O2k, O2k-FluoRespirometer
Link MitoPedia: DatLab, Oroboros O2k
Image
DatLab

DatLab 7: innovations

A DL7 version is available for beta-testing (2017-July).


Publications in the MiPMap
About Oroboros
DatLab 7: innovations.


Oroboros (2017-07-11) Mitochondr Physiol Network

Abstract: Gradl L, Capek O, Doerrier C, Sigl R, Mészáros A, Gnaiger E (2017) DatLab 7: innovations. Mitochondr Physiol Network 21.16

DatLab 7 is the newly developed software for high-resolution fluororespirometry (HRFR) incorporating the legacy of previous DatLab versions (high-resolution respirometry) and new, user-friendly features on quality control, documentation, and traceability of measurements with the Oroboros O2k.


O2k-Network Lab: AT_Innsbruck_Oroboros


Oroboros O2k data aquisition

DatLab 7 is optimized for the new Oroboros O2k-Series H applications, and supports all configurations of the previous O2k-Series (A-G). At the heart of DatLab 7, a conceptual unification is obtained in calibrations and flux analysis of the O2k-Fluorometer channels (oxygen and fluorescence: H2O2, mitochondrial membrane potential, calcium, ATP) and add-on O2k-Modules (ion selective electrodes: TTP+ and pH; amperometric modules: nitric oxide or hydrogen suflide).

Display and graphs

Graphs are further improved for real-time display and publication. Versatile options allow an optimal setup of the graphical display of data. Standard graph layouts are provided. Lab and User-defined graph layouts can be stored and organized to support specific applications.

Management of user accounts and experiments

As a new feature, user accounts are assigned to each measurement for better traceability and customization. This facilitates the organization of experimental data and allows user-specific configurations in DatLab 7. A detailed description section, cohort, sample and subsample data in the “Experimental setup” improves organization and analysis of results.

DL-Protocols

Besides keeping the flexibility to run user-defined experiments, DatLab 7 is now equipped with the new DL-Protocols feature. A collection of evaluated and tested DL-Protocols are available providing instructions for performing standardized experiments according to a selected substrate-uncoupler-inhibitor titration (SUIT) protocol or instrumental quality control protocols (calibration and instrumental background tests). The user is guided stepwise through the protocol (displayed in the DL-Protocol window), facilitating calibration steps, minimizing handling errors and greatly improving performance during O2k measurements. Moreover, an automatic warning at low oxygen levels can be set to remind the user to re-oxygenate the chamber in time. The DL-Protocol window displays the sequence of pre-programmed events with chemicals, titration volumes and corresponding final concentrations. These are saved automatically when the event is set. For protocol-specific marks the mark names are automatically assigned, with titration volumes which are used for calculation of sample dilution in analysis templates.

Events and marks

Concentration and volume of the chemicals added during Substrate-uncoupler-inhibition-titration (SUIT) protocols can be stored together with event names and comments. Automatic outlier detection helps to avoid unwanted data points when setting marks during respiratory steady-states. On top the user has the option to delete, interpolate or restore data points of marks.

Mark statistics

Numerous statistical functions are available for data analysis. “Marks statistics” organizes the marks and provides flexibility in displaying DL-Protocol marks, user-defined marks or both. The copy-to-clipboard function, with extended settings for backward compatibility, facilitates export to any third-party analysis software.

Experimental log

An Experimental log enables to view and generate detailed report of experimental-, software- and hardware settings for optimal tracking of each experiment.

Contextual help

A hyperlink-based contextual help system provides optimal support throughout settings and experimental steps in DatLab 7. MitoPedia provides the background for this function, which warranties up-to-date and verified information anytime.

TIP2k control

The software-driven, feedback-controlled Titration-Injection microPump (TIP2k) is an integral part of the O2k. In DatLab 7 TIP2k control has improved and facilitates user friendly and more flexible programming.


DatLab O2k-Manuals

  • These O2k-Manuals are for DatLab 6.
ChapterSectionLast update
MiPNet06.03 POS-calibration-SOP
O2k-Manual
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
2023-02-06
MiPNet12.10 TIP2k-manual
O2k-Manual
Titration-Injection microPump TIP2k manual.
2021-07-08
MiPNet14.06 Instrumental O2 background
O2k-Manual
O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux.
2023-10-19
MiPNet15.03 O2k-MultiSensor-ISE
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2021-08-02
MiPNet17.05 O2k-Fluo LED2-Module
O2k-Manual
O2k-Fluo LED2-Module.
2021-06-22
MiPNet19.01 O2k-Core Manual Series F DatLab 5
O2k-Manual
O2k-Core Manual - up to O2k-Series F and DatLab 5.
2014-03
MiPNet19.18 O2k-Series G Core manual
O2k-Manual
O2k-Core manual contents.
2016-08-08
MiPNet19.18A O2k-Series G: Start
O2k-Manual
Oxygraph-2k: start high-resolution respirometry.
2016-08-08
MiPNet19.18C DatLab 6: Guide
O2k-Manual
DatLab-guide through the menus.
2016-08-24
MiPNet19.18E O2 flux analysis - DatLab 6
O2k-Manual
Oxygen flux analysis: DatLab real-time.
2016-08-08
MiPNet22.11 O2k-FluoRespirometer manual
O2k-Manual
O2k-FluoRespirometer manual.
2023-03-14
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08
MiPNet24.14 O2k-sV-Module manual
O2k-Manual
O2k-sV-Module manual.
2020-03-17
MiPNet26.06 DatLab 7: Guide
O2k-Manual
DatLab-guide through the menus.
2021-06-02
» DatLab 2

Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



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MitoPedia: DatLab

MitoPedia: DatLab

» MitoPedia: DatLab


PC requirements

  • A PC or notebook is required (not included in our offers), connecting DatLab to the O2k via the USB cable supplied with the O2k.
  • Minimum requirements: Intel-Core-2 or equivalent CPU, 2GB RAM, Windows XP
  • Recommended: Intel i5 or equivalent CPU, 4GB RAM, Windows 7, SSD
  • Working recommendation: For data safety reasons and optimal DatLab performance it is strongly recommend to operate your DatLab files, DL-Protocols and DatLab templates on the PC‘s local HDD.



Install DatLab 6

» DatLab 6 innovations - OROBOROS INSTRUMENTS released DatLab 6 in October 2014.
For DatLab 5 users, the O2k-Core Manual for DatLab 5 is still available.

Download

  • From the OROBOROS USB-flash drive delivered with the O2k.
» O2k-Manual: MiPNet19.18A O2k-Start
  • O2k users can download the DatLab software from the OROBOROS website: » www.oroboros.at
  • The password is provided in the context of delivery of the O2k.
  • The Bioblast password is not the same as the Datlab password.
  • If the password got lost, contact: [email protected]


Install DatLab for two or more O2ks on one computer

  1. Install two (or more) copies of DatLab in two (or more) different directories, e.g. DatLab_O2k1 and DatLab_O2k2.
  2. Create two or more shortcuts to the different .exe files. (the .exe files do not have to be renamed because they are in different directories). In this way the O2k-specific .ini files are separated in the different directories.
  3. Assign different Power-O2k numbers (see MiPNet19.18A_O2k-Start) and activate Graph/Display Power-O2k (see MiPNet19.18C_DatLab_Guide) to distinguish the individual O2ks in the graphs.


Upgrade DatLab and compatibility

  1. DatLab can be installed within the directory of a previous DatLab version (recommended option). In this case all DatLab files will be kept, but some layouts are overwritten.
  2. During the first start of DatLab the information in any previous *.ini file in the same directory will be copied to the new *.ini.
  3. The nomenclature of some default graph layouts has changed. We recommend users upgrading from DatLab 5 to update Graph layouts after installing the new DatLab version.
  • Compatibility with DatLab 5: DatLab 6 is downwards compatible with DatLab 5: DatLab 6 can read all files generated by DatLab 5. DatLab 5 is upwards compatible with 6 in regards to basic data storage. However, new features in DatLab 6 may cause problems e.g. in Marks Statistics. We therefore do not recommend to open files created with DatLab 6 in DatLab 5.
  • Compatibility with DatLab 4: DatLab 6 is downwards compatible with DatLab 4: DatLab 6 can read all files generated by DatLab 4. However, DatLab 6 will save these files always in the DatLab 5/6 format. Afterwards, these files will no longer be readable by DatLab 4. Therefore, we recommend doing a complete transition from DatLab 4 to DatLab 6, upgrading all computers. However, there is no need to mass convert the old DatLab 4 files. They can stay in the DatLab 4 format and will be converted by DatLab 6 when necessary.
  • Talk:DatLab#DatLab_for_Apple


Information for IT-administrators

DatLab does not write to any part of the registry nor to any directory besides the program directory and its subdirectories, the data-directory and the users temporary files folder.


Labels: MiParea: Instruments;methods 





HRR: O2k-Manual 

O2k-Manual, O2k-Core, DatLab