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| {{MitoPedia | | {{MitoPedia |
| |abbr=CII, CII-linked, SRot, S | | |abbr=SRot, S |
| |description='''CII-linked; SRot''': '''[[Succinate]]''' (S) supports electron flux through [[Complex II]] via flavin adenine dinucleotide (FADH2) into the membrane-bound [[electron transfer system]]. Inhibition of Complex I by '''[[rotenone]]''' (Rot; or amytal, piericidine) prevents accumulation of [[Oxaloacetate|oxaloacetate]] which is a potent inhibitor of [[succinate dehydrogenase]]. After inhibition of CI by [[rotenone]], the NADH-linked dehydrogenases become inhibited by the redox shift from NAD<sup>+</sup> to NADH. Succinate dehydrogenase is activated by succinate and ATP, which explains in part the time-dependent increase of respiration in isolated mitochondria after addition of rotenone (first), succinate and ADP. | | |description=''See'' '''[[S-pathway control state]] (previous: CII-linked) |
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| The '''Complex II-linked substrate state''' is induced in mt-preparations by addition of [[succinate]]&[[rotenone]] (Complex I inhibitor). Succinate is the direct substrate of [[Complex II]] (succinate dehydrogenase). In CII-linked respiration, only Complex III and Complex IV are involved in pumping protons from the matrix (P-phase) to the N-phase with a ~P/O ratio of 1.75 (P/O<sub>2</sub> = 3.5).
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| |info=[[Gnaiger 2014 MitoPathways |Gnaiger 2014 MitoPathways - Chapter 4.1]], [[Gnaiger 2000 Proc Natl Acad Sci U S A]]
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| }} | | }} |
| {{MitoPedia methods}} | | {{MitoPedia concepts |
| {{MitoPedia SUIT
| | |mitopedia concept=Respiratory state, SUIT state, Find |
| |mitopedia SUIT=SUIT state | |
| }} | | }} |
| {{MitoPedia topics | | {{MitoPedia topics |
| |mitopedia topic=Respiratory state, Substrate and metabolite | | |mitopedia topic=EAGLE |
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| [[File:S(Rot).jpg|400px|thumb|Succinate+rotenone, S(Rot). From [[Gnaiger 2014 MitoPathways]].]]
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| == SRot(L) ==
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| # ~P/O2 ratio: [[Gnaiger 2000 Proc Natl Acad Sci U S A]]
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| # [[O2k-Protocols]]: [[MiPNet12.11 MitoRespiration]]
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| # Comparison with [[S alone]], CI<small>&</small>II, and H<sub>2</sub>O<sub>2</sub> production: [[Makrecka-Kuka 2015 Biomolecules]]
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| == SRot(P) ==
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| # ~P/O2 ratio: [[Gnaiger 2000 Proc Natl Acad Sci U S A]]
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| # [[O2k-Protocols]]: [[MiPNet12.11 MitoRespiration]]
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| # Comparison with [[S alone]] CI<small>&</small>II, and H<sub>2</sub>O<sub>2</sub> production: [[Makrecka-Kuka 2015 Biomolecules]]
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| == SRot(E) ==
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| # [[O2k-Protocols]]: [[MiPNet12.11 MitoRespiration]]
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| # Comparison with CI-CI<small>&</small>II-CII SUIT protocol: [[Votion 2012 PLoS One]]
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| # [[SUIT-RP1]]_'''7Rot''': 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S '''<u>7Rot</u>''' 8Gp 9Ama 10Tm 11Azd
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| == List of publications: CII-linked substrate state ==
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| : This list does not distinguish between substrate states for [[Succinate alone]] (without Rot) and SRot.
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| {{#ask:[[Category:Publications]] [[Substrate states::CII]]
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| |?Was published in year=Year
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| |?Has title=Reference
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| |?Mammal and model
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| |?Tissue and cell
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| |?Stress
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| |?Diseases
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| |format=broadtable
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| |limit=5000
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| |offset=0
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| |sort=Was published in year
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| |order=descending
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| }} | | }} |