Difference between revisions of "Chou 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
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|abstract='''Protocol''': final concentration of digitonin in chamber is 10μg/ml | |abstract='''Protocol''': final concentration of digitonin in chamber is 10μg/ml | ||
cell number in chamber is 2 millions cell/ml, cell type PBMC, Malate (2mM), Palmitoyl-DLcarnitine-HCl (20μM), ADP (2.5mM), pyruvate (5mM), glutamate (10mM), succinate (10mM), rotenone (0.1μM), malonic acid(5mM), myxothiazol (0.5μM), antimycin A (2.5μM), TMPD (0.5mM), azide (100mM) | cell number in chamber is 2 millions cell/ml, cell type PBMC, Malate (2mM), Palmitoyl-DLcarnitine-HCl (20μM), ADP (2.5mM), pyruvate (5mM), glutamate (10mM), succinate (10mM), rotenone (0.1μM), malonic acid(5mM), myxothiazol (0.5μM), antimycin A (2.5μM), TMPD (0.5mM), azide (100mM) | ||
|keywords=PBMC | |||
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{{Labeling | {{Labeling | ||
Revision as of 08:10, 25 October 2016
| Chou 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Chou Cheng-Hsien (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
Protocol: final concentration of digitonin in chamber is 10μg/ml cell number in chamber is 2 millions cell/ml, cell type PBMC, Malate (2mM), Palmitoyl-DLcarnitine-HCl (20μM), ADP (2.5mM), pyruvate (5mM), glutamate (10mM), succinate (10mM), rotenone (0.1μM), malonic acid(5mM), myxothiazol (0.5μM), antimycin A (2.5μM), TMPD (0.5mM), azide (100mM)
• Keywords: PBMC
Labels:
Tissue;cell: Blood cells Preparation: Permeabilized cells, Permeabilized tissue
Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Palmitoylcarnitine
