Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum: Difference between revisions
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|year=2015 | |year=2015 | ||
|event=Fatty Acid Oxidation O2k-Network Discussion Forum | |event=Fatty Acid Oxidation O2k-Network Discussion Forum | ||
|abstract=*Saponin permeabilized skeletal muscle fiber bundles | |||
*'''Protocol''': | |||
#0.5mM Malate | |||
#50 µM palmitoyl-CoA + 2mM carnitine | |||
#5mM ADP | |||
#From here on out, various combinations for titration protocol | |||
*Coupling states: | |||
#LEAK_M+Palmitoylcarnitine | |||
#P_M+Palmitoylcarnitine | |||
#P_M+Palmitoylcarnitine+S | |||
#E_O+CCCP titrations | |||
#E_S+Rot | |||
#ROX_AntimycinA | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Human, Mouse | |||
|tissues=Skeletal muscle | |||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|substratestates=FAO | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII, FAO, CI&II, ROX | |||
|additional=Palmitoylcarnitine | |additional=Palmitoylcarnitine | ||
}} | }} | ||
Revision as of 09:23, 4 December 2015
| Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Cannon DT (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
- Saponin permeabilized skeletal muscle fiber bundles
- Protocol:
- 0.5mM Malate
- 50 µM palmitoyl-CoA + 2mM carnitine
- 5mM ADP
- From here on out, various combinations for titration protocol
- Coupling states:
- LEAK_M+Palmitoylcarnitine
- P_M+Palmitoylcarnitine
- P_M+Palmitoylcarnitine+S
- E_O+CCCP titrations
- E_S+Rot
- ROX_AntimycinA
Labels:
Organism: Human, Mouse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Palmitoylcarnitine
