Braz-Mota 2018 Sci Total Environ

[[Has title::Braz-Mota S, Campos DF, MacCormack TJ, Duarte RM, Val AL, Almeida-Val VMF (2018) Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi). Sci Total Environ 630:1168-80.]]

» [[Has info::PMID: 29554738]]

Was written by::Braz-Mota S, Was written by::Campos DF, Was written by::MacCormack TJ, Was written by::Duarte RM, Was written by::Val AL, Was written by::Almeida-Val VMF (Was published in year::2018) Was published in journal::Sci Total Environ

Abstract: [[has abstract::Copper oxide nanoparticles (nCuO) are widely used in boat antifouling paints and are released into the environment, potentially inducing toxicity to aquatic organisms. The present study aimed to understand the effects of nCuO and dissolved copper (Cu) on two ornamental Amazon fish species: dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi). Fish were exposed to 50% of the LC₅₀ for nCuO (dwarf cichlid 58.31μgL^-1 and cardinal tetra 69.6μgL^-1) and Cu (dwarf cichlid 20μgL^-1 and cardinal tetra 22.9μgL^-1) for 24, 48, 72 and 96h. Following exposure, aerobic metabolic rate (ṀO₂), gill osmoregulatory physiology and mitochondrial function, oxidative stress markers, and morphological damage were evaluated. Our results revealed species specificity in metabolic stress responses. An increase of ṀO₂ was noted in cardinal tetra exposed to Cu, but not nCuO, whereas ṀO₂ in dwarf cichlid showed little change with either treatment. In contrast, mitochondria from dwarf cichlid exhibited increased proton leak and a resulting decrease in respiratory control ratios in response to nCuO and Cu exposure. This uncoupling was directly related to an increase in reactive oxygen species (ROS) levels. Our findings reveal different metabolic responses between these two species in response to nCuO and Cu, which are probably caused by the differences between species natural histories, indicating that different mechanisms of toxic action of the contaminants are associated to differential osmoregulatory strategies among species.]] • Keywords: has publicationkeywords::CuO nanoparticles, has publicationkeywords::Metabolic rate, has publicationkeywords::Mitochondria respiration, has publicationkeywords::Ornamental Amazon fishes, has publicationkeywords::Oxidative stress, has publicationkeywords::Reactive oxygen species • Bioblast editor: [[has editor::Kandolf G]] • O2k-Network Lab: Was published by MiPNetLab::BR Manaus Val AL

Labels: MiParea: MiP area::Respiration, MiP area::Pharmacology;toxicology 

Stress:Injury and adaptation::Oxidative stress;RONS  Organism: Organism::Fishes  Tissue;cell: tissue and cell::Lung;gill 

Coupling state: Coupling states::LEAK, Coupling states::OXPHOS, Coupling states::ET  Pathway: Pathways::N, Pathways::NS, Pathways::ROX  HRR: Instrument and method::Oxygraph-2k 

additional label::Labels, additional label::2018-04