Braz-Mota 2017 Thesis: Difference between revisions
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|abstract=Copper oxide nanoparticles (nCuO) are widely used in boat antifouling paints, and are released into the environment, inducing toxicity to aquatic organisms. The present study aimed to understand the effects of nCuO and dissolved copper on two ornamental Amazon fish species: ''Apistogramma agassizii'' and ''Paracheirodon axelrodi''. Fish were exposed to 50% nCuO LC<sub>50</sub> (''A. agassizii'' 375 ฮผg L<sup>-1</sup> and ''P. axelrodi'' 460 ฮผg L-1) and 50% Cu LC<sub>50</sub> (''A. agassizii'' 20 mg L<sup>-1</sup> and ''P. axelrodi'' 22.9 ฮผg L-1) for 24, 48, 72 and 96 hours. Metabolic rate (MO<sub>2</sub>), gill osmorregulatory processes, gill mitochondria oxidative phosphorylation capacity and ROS generation, oxidative stress defense and morfological damages were evaluated. Our results showed a strong increase in MO<sub>2</sub> and a higher impairment in its gillโs morphology in ''P. axelrodi'' after the copper exposures. Differently, ''A. agassizii'' presented an increased proton leak (i.e. uncoupling between respiration and ATP production) in response to nCuO and Cu exposure, thus decreasing their Respiratory Control Rate (RCR). Interestingly, this uncoupling was directly related to an increase in ROS levels. Our findings reveal that the metabolic responses of these two species in response to nCuO and Cu, which are probably caused by the differences between species natural histories, indicating that different mechanisms of toxic action of the contaminants are associated to differences in the sensibility of these two species. | |abstract=Copper oxide nanoparticles (nCuO) are widely used in boat antifouling paints, and are released into the environment, inducing toxicity to aquatic organisms. The present study aimed to understand the effects of nCuO and dissolved copper on two ornamental Amazon fish species: ''Apistogramma agassizii'' and ''Paracheirodon axelrodi''. Fish were exposed to 50% nCuO LC<sub>50</sub> (''A. agassizii'' 375 ฮผg L<sup>-1</sup> and ''P. axelrodi'' 460 ฮผg L-1) and 50% Cu LC<sub>50</sub> (''A. agassizii'' 20 mg L<sup>-1</sup> and ''P. axelrodi'' 22.9 ฮผg L-1) for 24, 48, 72 and 96 hours. Metabolic rate (MO<sub>2</sub>), gill osmorregulatory processes, gill mitochondria oxidative phosphorylation capacity and ROS generation, oxidative stress defense and morfological damages were evaluated. Our results showed a strong increase in MO<sub>2</sub> and a higher impairment in its gillโs morphology in ''P. axelrodi'' after the copper exposures. Differently, ''A. agassizii'' presented an increased proton leak (i.e. uncoupling between respiration and ATP production) in response to nCuO and Cu exposure, thus decreasing their Respiratory Control Rate (RCR). Interestingly, this uncoupling was directly related to an increase in ROS levels. Our findings reveal that the metabolic responses of these two species in response to nCuO and Cu, which are probably caused by the differences between species natural histories, indicating that different mechanisms of toxic action of the contaminants are associated to differences in the sensibility of these two species. | ||
|editor=[[Kandolf G]], | |editor=[[Kandolf G]], | ||
|mipnetlab=BR Manaus Val AL | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
| Line 13: | Line 14: | ||
|tissues=Lung;gill | |tissues=Lung;gill | ||
|preparations=Homogenate | |preparations=Homogenate | ||
|couplingstates=LEAK, OXPHOS, | |couplingstates=LEAK, OXPHOS, ET | ||
|pathways=N, NS, ROX | |pathways=N, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Labels, 2017-10, Ampliflu Red | |additional=Labels, 2017-10, Ampliflu Red | ||
}} | }} | ||
Latest revision as of 17:02, 9 November 2017
| Braz Mota S (2017) Estudo comparativo da toxicidade e dos mecanismos de aรงรฃo tรณxica de nanopartรญculas de cobre e cobre em duas espรฉcies de peixes da Amazรดnia: Apistogramma agassizii e Paracheirodon axelrodi. Dissertation p.78. |
ยป Open Access
Braz-Mota S (2017) Dissertation
Abstract: Copper oxide nanoparticles (nCuO) are widely used in boat antifouling paints, and are released into the environment, inducing toxicity to aquatic organisms. The present study aimed to understand the effects of nCuO and dissolved copper on two ornamental Amazon fish species: Apistogramma agassizii and Paracheirodon axelrodi. Fish were exposed to 50% nCuO LC50 (A. agassizii 375 ฮผg L-1 and P. axelrodi 460 ฮผg L-1) and 50% Cu LC50 (A. agassizii 20 mg L-1 and P. axelrodi 22.9 ฮผg L-1) for 24, 48, 72 and 96 hours. Metabolic rate (MO2), gill osmorregulatory processes, gill mitochondria oxidative phosphorylation capacity and ROS generation, oxidative stress defense and morfological damages were evaluated. Our results showed a strong increase in MO2 and a higher impairment in its gillโs morphology in P. axelrodi after the copper exposures. Differently, A. agassizii presented an increased proton leak (i.e. uncoupling between respiration and ATP production) in response to nCuO and Cu exposure, thus decreasing their Respiratory Control Rate (RCR). Interestingly, this uncoupling was directly related to an increase in ROS levels. Our findings reveal that the metabolic responses of these two species in response to nCuO and Cu, which are probably caused by the differences between species natural histories, indicating that different mechanisms of toxic action of the contaminants are associated to differences in the sensibility of these two species.
โข Bioblast editor: Kandolf G โข O2k-Network Lab: BR Manaus Val AL
Labels: MiParea: Respiration, Comparative MiP;environmental MiP, Pharmacology;toxicology
Organism: Fishes
Tissue;cell: Lung;gill
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET
Pathway: N, NS, ROX
HRR: Oxygraph-2k
Labels, 2017-10, Ampliflu Red
