Bellance 2009 Int J Biochem Cell Biol

[[Has title::Bellance N, Benard G, Furt F, Begueret H, Smolková K, Passerieux E, Delage JP, Baste JM, Moreau P, Rossignol R (2009) Bioenergetics of lung tumors: Alteration of mitochondrial biogenesis and respiratory capacity. Int J Biochem Cell Biol 41:2566-77.]]

» [[Has info::PMID: 19712747]]

Was written by::Bellance N, Was written by::Benard G, Was written by::Furt F, Was written by::Begueret H, Was written by::Smolkova K, Was written by::Passerieux E, Was written by::Delage JP, Was written by::Baste JM, Was written by::Moreau P, Was written by::Rossignol R (Was published in year::2009) Was published in journal::Int J Biochem Cell Biol

Abstract: [[has abstract::Little is known on the metabolic profile of lung tumors and the reminiscence of embryonic features. Herein, we determined the bioenergetic profiles of human fibroblasts taken from lung epidermoid carcinoma (HLF-a) and fetal lung (MRC5). We also analysed human lung tumors and their surrounding healthy tissue from four patients with adenocarcinoma. On these different models, we measured functional parameters (cell growth rates in oxidative and glycolytic media, respiration, ATP synthesis and PDH activity) as well as compositional features (expression level of various energy proteins and upstream transcription factors). The results demonstrate that both the lung fetal and cancer cell lines produced their ATP predominantly by glycolysis, while oxidative phosphorylation was only capable of poor ATP delivery. This was explained by a decreased mitochondrial biogenesis caused by a lowered expression of PGC1α (as shown by RT-PCR and Western blot) and mtTFA. Consequently, the relative expression of glycolytic versus OXPHOS markers was high in these cells. Moreover, the re-activation of mitochondrial biogenesis with resveratrol induced cell death specifically in cancer cells. A consistent reduction of mitochondrial biogenesis and the subsequent alteration of respiratory capacity was also observed in lung tumors, associated with a lower expression level of bcl2. Our data give a better characterization of lung cancer cells' metabolic alterations which are essential for growth and survival. They designate mitochondrial biogenesis as a possible target for anti-cancer therapy.]] • Keywords: has publicationkeywords::Lung tumors, has publicationkeywords::Bioenergetics, has publicationkeywords::Mitochondria, has publicationkeywords::Oxidative phosphorylation

• O2k-Network Lab: Was published by MiPNetLab::CZ Prague Jezek P, Was published by MiPNetLab::FR Bordeaux Rossignol R

Labels: MiParea: MiP area::Respiration, MiP area::mt-Biogenesis;mt-density, MiP area::Genetic knockout;overexpression, MiP area::mt-Medicine, MiP area::Pharmacology;toxicology  Pathology: Diseases::Cancer 

Organism: Organism::Human  Tissue;cell: tissue and cell::Fibroblast  Preparation: Preparation::Intact organ, Preparation::Intact cells 

Regulation: Topic::ADP, Topic::Substrate  Coupling state: Coupling states::OXPHOS 

HRR: Instrument and method::Oxygraph-2k