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Difference between revisions of "BAM15"

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{{MitoPedia
{{MitoPedia
|abbr=DNP
|abbr=DNP

Revision as of 14:00, 21 May 2014


high-resolution terminology - matching measurements at high-resolution


BAM15

Description

2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.

Abbreviation: DNP

Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol



MitoPedia topics: Uncoupler 

Preparation of 10 mM DNP stock solution (dissolved in H2O):

Caution: Toxic!

1. Weigh 3.7 mg of DNP (FW = 184.11).
2. Dissolve in 1.2 ml H2O.
3. Neutralize with 1 N KOH.
4. Adjust final volume to 2 ml and divide into 0.2 ml portions.
6. Store at -20 Β°C.