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Difference between revisions of "Azide"

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::* Final concentration: โ‰ฅ100 mM.
::* Final concentration: โ‰ฅ100 mM.


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Revision as of 15:02, 3 September 2020


high-resolution terminology - matching measurements at high-resolution


Azide

Description

Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).

Abbreviation: Azd

Reference: MiPNet03.02

Application in HRR

Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; FW = 65.01.

Caution: Very toxic!


Preparation of 4 M storage and stock solution (dissolved in H2O):

1) Weigh 260 mg of sodium azide.
2) Dissolve in 1 mL H20.
3) Divide into 0.5 mL portions.
4) Store at -20 ยฐC.


O2k manual titrations MiPNet09.12 O2k-Titrations

  • Titration volume: โ‰ฅ50 ยตL using a 100 ยตL syringe (2 mL O2k-Chamber).
  • Final concentration: โ‰ฅ100 mM.




MitoPedia topics: Inhibitor