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Difference between revisions of "Assay"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=n.a.
|description=An experimental '''assay''' is a test run on a single [[sample]]. An assay may have ''n'' [[repetition]]s for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. An [[assay series]] consists of replications of ''N'' assays on different samples.
|description=An experimental '''assay''' is a test run on a single [[sample]]. An assay may have ''n'' [[repetition]]s for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. An [[assay series]] consists of replications of ''N'' assays on different samples.
}}
}}

Revision as of 20:47, 27 January 2016


high-resolution terminology - matching measurements at high-resolution


Assay

Description

An experimental assay is a test run on a single sample. An assay may have n repetitions for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. An assay series consists of replications of N assays on different samples.


MitoPedia concepts: "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property. MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 



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