Difference between revisions of "Antimycin A"
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:::: '''Preparation of 5 mM stock solution''' (dissolved in | :::: '''Preparation of 5 mM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''': | ||
::::# Weigh 5.4 mg of antimycin A into a small glass vial. | ::::# Weigh 5.4 mg of antimycin A into a small glass vial. | ||
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:::: '''Preparation of 1,25 mM stock solution''' (dissolved in | :::: '''Preparation of 1,25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-chamber''': | ||
::::# Take 100 µL of the 5 mM stock solution. | ::::# Take 100 µL of the 5 mM stock solution. | ||
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:::'''Microalgae applications''' | :::'''Microalgae applications''' | ||
::: For the specific case of microalgal cells it is recommended to prepare higher concentration: | ::: For the specific case of microalgal cells it is recommended to prepare higher concentration: | ||
:::: '''Preparation of 10 mM stock solution''' (dissolved in | :::: '''Preparation of 10 mM stock solution''' (dissolved in ethanol abs.) | ||
::::# Weigh 10.8 mg of antimycin A and dissolve in 2 mL of ethanol | ::::# Weigh 10.8 mg of antimycin A and dissolve in 2 mL of ethanol abs.. | ||
::::# Divide into 0.2 mL portions in clear glass vials. | ::::# Divide into 0.2 mL portions in clear glass vials. | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. |
Revision as of 11:54, 22 June 2021
Description
Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor). High concentrations of antimycin A also inhibit acyl-CoA oxidase and D-amino acid oxidase.
Abbreviation: Ama
Reference: MiPNet09.12
Application in HRR
- Ama: Antimycin A (from Streptomyces sp.; ), Sigma-Aldrich: A8674, store at -20 °C, CAS: 1397-94-0, M = 540 g·mol-1
- Hazard statements: H300, H400; fatal if swallowed, very toxic to aquatic life
- Ama: Antimycin A (from Streptomyces sp.; ), Sigma-Aldrich: A8674, store at -20 °C, CAS: 1397-94-0, M = 540 g·mol-1
- Sigma recommends to store dessicated, there is no hint to light sensitivity.
- Light sensitivity of Antimycin A is reported by another vendor: AlfaAesar.
- Preparation of 5 mM stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- Weigh 5.4 mg of antimycin A into a small glass vial.
- Add 2 mL ethanol.
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 2.5 µM.
- Preparation of 1,25 mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- Take 100 µL of the 5 mM stock solution.
- Add 300 µL ethanol.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 2.5 µM.
- Microalgae applications
- For the specific case of microalgal cells it is recommended to prepare higher concentration:
- Preparation of 10 mM stock solution (dissolved in ethanol abs.)
- Weigh 10.8 mg of antimycin A and dissolve in 2 mL of ethanol abs..
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 8 µL using a 10 µL syringe.
- Final concentration: 40 µM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
Antimycin A and ROS production
- Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]).
Inhibition of acyl-CoA oxidase and D-amino acid oxidase
- Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - »Bioblast link«
MitoPedia topics:
Inhibitor