Difference between revisions of "Amytal"
From Bioblast
| Line 16: | Line 16: | ||
'''Preparation of 200 mM stock solution''' (dissolved in 99.9% Ethanol): | '''Preparation of 200 mM stock solution''' (dissolved in 99.9% Ethanol): | ||
::1) weigh | ::1) weigh 49.65 mg of Amytal | ||
::2) dissolve in 10 ml 50% ethanol | ::2) dissolve in 10 ml 50% ethanol | ||
::3) divide into 500 µl aliquots (use dark glass vials - light sensitive) | ::3) divide into 500 µl aliquots (use dark glass vials - light sensitive) | ||
Revision as of 13:16, 16 May 2012
- high-resolution terminology - matching measurements at high-resolution
Amytal
Description
Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is and barbiturate drug and an inhibitor of Complex I. TOXIC!
Abbreviation: Amy
MitoPedia topics:
Inhibitor
Application in HRR
Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; FW = 248.25
Preparation of 200 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 49.65 mg of Amytal
- 2) dissolve in 10 ml 50% ethanol
- 3) divide into 500 µl aliquots (use dark glass vials - light sensitive)
- 4) store frozen at -20 °C
Comment:
The product is withdrawn from Sigma but some residual amount is still available.
