Difference between revisions of "Amytal"

Revision as of 13:15, 16 May 2012

high-resolution terminology - matching measurements at high-resolution

Amytal

Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is and barbiturate drug and an inhibitor of Complex I. TOXIC!

Abbreviation: Amy

MitoPedia topics: Inhibitor

Amy: Amytal (Amytal sodium salt, C₁₁H₁₇N₂O₃Na); Sigma A 4430, 50 g; FW = 248.25

Preparation of 200 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 0.497 g of Amytal

2) dissolve in 10 ml 50% ethanol

3) divide into 500 µl aliquots (use dark glass vials - light sensitive)

4) store frozen at -20 °C

Comment: The product is withdrawn from Sigma but some residual amount is still available.

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|description=Inhibitor of [[Complex I]]; barbiturate drug.
+|abbr=Amy
+|description= Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is and barbiturate drug and an inhibitor of [[Complex I]]. '''TOXIC!'''
 |type=Inhibitor ETS
 }}
@@ Line 7: / Line 8: @@
 |type=Inhibitor ETS
 }}
+== Application in [[HRR]] ==
+'''Amy: Amytal'''  (Amytal sodium salt,  C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na);  Sigma A 4430, 50 g; FW = 248.25
+'''Preparation of 200 mM stock solution''' (dissolved in 99.9% Ethanol):
+::1) weigh 0.497 g of Amytal
+::2) dissolve in 10 ml 50% ethanol
+::3) divide into 500 µl aliquots (use dark glass vials - light sensitive)
+::4) store frozen at -20 °C
+'''Comment:'''
+''The product is withdrawn from Sigma but some residual amount is still available.''