Difference between revisions of "Amytal"
From Bioblast
Fontana Mona (talk | contribs) |
(replaced 99.9 % ethanol by ethanol abs.) |
||
| (6 intermediate revisions by 4 users not shown) | |||
| Line 4: | Line 4: | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | }} | ||
__TOC__ | |||
== Application in [[HRR]] == | |||
:::'''Amy: Amytal''' (Amytal sodium salt, C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na); Sigma A 4430, 50 g; M = 248.25 g·mol<sup>-1</sup>. ''The product is withdrawn from Sigma but some residual amounts are still available.'' | |||
::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | |||
::::'''Preparation of 200 mM stock solution''' (dissolved in ethanol abs.): | |||
::::# Weigh 49.65 mg of amytal. | |||
::::# Dissolve in 10 mL 50% ethanol. | |||
::::# Divide into 500 µL aliquots (use dark glass vials - light sensitive). | |||
::::# Store at -20 °C. | |||
{{MitoPedia methods|type=Inhibitor ETS | {{MitoPedia methods|type=Inhibitor ETS | ||
}} | }} | ||
| Line 10: | Line 24: | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | }} | ||
Latest revision as of 11:53, 22 June 2021
- high-resolution terminology - matching measurements at high-resolution
Amytal
Description
Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of Complex I.
Abbreviation: Amy
Application in HRR
- Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; M = 248.25 g·mol-1. The product is withdrawn from Sigma but some residual amounts are still available.
- Caution: Light sensitive and toxic!
- Preparation of 200 mM stock solution (dissolved in ethanol abs.):
- Weigh 49.65 mg of amytal.
- Dissolve in 10 mL 50% ethanol.
- Divide into 500 µL aliquots (use dark glass vials - light sensitive).
- Store at -20 °C.
MitoPedia topics:
Inhibitor
