Difference between revisions of "Amytal"

Latest revision as of 11:53, 22 June 2021

high-resolution terminology - matching measurements at high-resolution

Amytal

Description

Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of Complex I.

Abbreviation: Amy

Application in HRR

Amy: Amytal (Amytal sodium salt, C₁₁H₁₇N₂O₃Na); Sigma A 4430, 50 g; M = 248.25 g·mol^-1. The product is withdrawn from Sigma but some residual amounts are still available.

Caution: Light sensitive and toxic!

Preparation of 200 mM stock solution (dissolved in ethanol abs.):

Weigh 49.65 mg of amytal.
Dissolve in 10 mL 50% ethanol.
Divide into 500 µL aliquots (use dark glass vials - light sensitive).
Store at -20 °C.

MitoPedia topics: Inhibitor

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Amy
-|description= Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of [[Complex I]].
+|description='''Amytal''' sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of [[Complex I]].
-|type=Inhibitor ETS
-}}
-{{MitoPedia topics
-|mitopedia topic=Inhibitor
 |type=Inhibitor ETS
 }}
+__TOC__
 == Application in [[HRR]] ==
-'''Amy: Amytal'''  (Amytal sodium salt,  C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na);  Sigma A 4430, 50 g; FW = 248.25
+:::'''Amy: Amytal'''  (Amytal sodium salt,  C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na);  Sigma A 4430, 50 g; M = 248.25 g·mol<sup>-1</sup>. ''The product is withdrawn from Sigma but some residual amounts are still available.''
-<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span>
+::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span>
+::::'''Preparation of 200 mM stock solution''' (dissolved in ethanol abs.):
-'''Preparation of 200 mM stock solution''' (dissolved in 99.9% Ethanol):
+::::# Weigh 49.65 mg of amytal.
+::::# Dissolve in 10 mL 50% ethanol.
-::1) weigh 49.65 mg of Amytal
+::::# Divide into 500 µL aliquots (use dark glass vials - light sensitive).
-::2) dissolve in 10 ml 50% ethanol
+::::# Store at -20 °C.
-::3) divide into 500 µl aliquots (use dark glass vials - light sensitive)
+{{MitoPedia methods|type=Inhibitor ETS
-::4) store frozen at -20 °C
+}}
+{{MitoPedia topics
+|mitopedia topic=Inhibitor
-'''Comment:'''
+|type=Inhibitor ETS
-''The product is withdrawn from Sigma but some residual amounts are still available.''
+}}