Amoedo 2012 Abstract Bioblast

Amoedo ND (2012) ... Mitochondr Physiol Network 17.12.

Link: MiPNet17.12 Bioblast 2012 - Open Access

Amoêdo, N.D., Rodrigues, M.F., Pereira, S.A.S., Melo, F.H., Jasiulionis, M.G., Galina A., Rumjanek, F.D. (2012)

Event: Bioblast 2012

The classic bioenergetic phenotype of cancer cells, an enhanced glycolysis with concomitant production of lactate even in high oxygen tension, was described by Otto Warburg approximately 90 years ago. However, the Warburg hypothesis does not necessarily imply in mitochondrial dysfunction. Current thinking considers tumor cells as adapted to an oxygen gradient within the tumor mass. Those cells exposed to oxygen will utilize the gas and those found in hypoxic regions of the tumor will adjust by means of metabolic symbiosis. Tumor cells exhibit accelerated growth as well as metastasis, two major events that must be supported by sufficient energy supply. This can be translated as metabolic reprogramming that up-regulates pathways that ultimately increase the rate of ATP production, synthesis of lipids and redox balance. According to Smolková et al. the process of carcinogenesis is guided by waves of gene expression that promote these metabolic changes. The energy metabolism of cancer cells is very heterogeneous. Indeed not all tumor cells display a high glycolytic flux as proposed by Warburg. Similarly, not all cancer cells grow fast and show intense anabolism. Furthermore, progression to metastasis appears to require mitochondrial function, an hypothesis that is compatible with the results obtained by our group. In order to show this we resorted to a murine model of melanoma. In this model a melanocyte cell line was subjected to several cycles of adhesion impediment, which produced stable cell lines exhibiting phenotypes corresponding to a progression from non-tumorigenic to metastatic cells. The different stages of malignant transformation were as follows: Non-tumorigenic cells melan-a (ma) (original murine melanocytes); non-tumorigenic cell line 4C (obtained after 4 cycles of adherence abrogation); non-metastatic 4C11- and metastatic 4C11+ melanoma cell lines, which were obtained by diluting the cells from the spheroids of the 4C cell line. The metabolic profile of each of these different cell lines was investigated by evaluating enzyme activities and expression of members of the glycolytic and oxidative pathways. Our results showed that metastatic cell line (4C11+) released the highest amounts of lactate and exhibited high LDH activity, typical of the Warburg effect. In contrast, results obtained with high-resolution respirometry with 4C11+ intact cells showed an increased oxidative metabolism, with enhanced rates of oxygen consumption coupled to ATP synthesis when compared to the other pre-malignant stages. Moreover we observed an increase in succinate dehydrogenase (Complex II) activity in these cells. Concomitantly, we detected an increase in activity of the electron transport system (ETS capacity). We did not observe an increase in mitochondrial content, mitochondrial biogenesis, nor alterations in fusion and fission process. These results suggest enhanced OXPHOS. This was thought to be associated to metastasis, a condition which would benefit from unrestricted supply of oxygen. Detailed analysis of patterns in this and other models of tumor progression may reveal whether the modulation of the oxidative metabolism is a feature of the metastatic process.

• Keywords: Cancer, tumor progression, mitochondrial function

• O2k-Network Lab: Laboratory of Biochemistry and Molecular Biology of Cancer - Professor Franklin Rumjanek

Labels:

Stress:Cancer; Apoptosis; Cytochrome c"Cancer; Apoptosis; Cytochrome c" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.  Organism: Mouse 

Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.  Enzyme: Complex I, Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.  Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Aerobic and Anaerobic Metabolism"Aerobic and Anaerobic Metabolism" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.  Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Theory 

1 Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. 2 Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil