MitoPedia alert

MitoPedia alert - 2024

A collection from the »Bioblast Agenda«

May 2024

• May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization? It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. - »Digitonin«, »SUIT-010«  - communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

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Apr 2024

• Apr-22 Mo

How can I evaluate the effect of one specific titration of a complex SUIT protocol? Flux control efficiencies express the change in respiration after a single step, in which there is a change in a metabolic control variable. - »Flux control efficiency«, »Metabolic control variable« - communicated by »Cardoso Luiza« and »Baglivo Eleonora«

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• Apr-10 We

Is fatty acid oxidation (FAO) considered as CI- or CII-driven respiration? Neither. There is simultaneous electron transfer from FAO into the Q-junction through the electron transferring flavoprotein dehydrogenase complex (CETFDH) and Complex I. - »Fatty acid oxidation«, »Gnaiger 2020 BEC MitoPathways«, »Gnaiger 2024 J Biol Chem«  - communicated by »Timon-Gomez Alba« and »Willis Jaime R«

Mar 2024

• Mar-25 Mo

Living cells or intact cells? Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury. - »Living cells«, »BEC 2020.1«, »Gnaiger 2020 BEC MitoPathways«  - communicated by »Leo Elettra« and »Timon-Gomez Alba«

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• Mar-11 Mo

Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O2] regime? Long diffusion distances in the pfi result in an O2 diffusion limitation, which is overcome by increasing the O2 partial pressure inside the chamber. Proper instrumental background correction is a must and O2 dependence of ROS production should be considered. - »Permeabilized muscle fibers«, »Oxygen flux - instrumental background«  - communicated by »Grings Mateus« and »Cardoso Luiza«

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Feb 2024

• Feb-26 Mo

ATP synthase = phosphorylation system? No, the phosphorylation system consists of adenine nucleotide translocase, phosphate carrier, and ATP synthase, which carry out the steps in the phosphorylation pathway. - »Phosphorylation pathway«, »Mitochondrial Pathways: Chapter 2.2«  - communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

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• Feb-12 Mo

What is the O2 solubility factor of the medium (FM) and why is it important? FM expresses the effect of the salt concentration on oxygen solubility relative to pure water, and varies depending on the temperature and composition of the medium. The correct FM needs to be entered in DatLab when performing an air calibration. - »Oxygen solubility factor« , »Air calibration in DatLab«  - communicated by »Baglivo Eleonora« and »Timon-Gomez Alba«

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Jan 2024

• Jan-31 We

Can the S-pathway O2 flux be derived by subtracting N-pathway O2 flux from NS-pathway O2 flux? No, the sum of the O2 fluxes measured in the presence of NADH-linked substrates alone and succinate as the single substrate is typically higher than using a combination of all these substrates. This effect is described as incomplete additivity: - »Additive effect of convergent electron flow«  - communicated by »Cardoso Luiza« and »Grings Mateus«

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• Jan-02 Tu

New year, new state? What is REN state? REN state is now distinguished from ROX state: REN is measured in mitochondrial preparations without addition of substrates, whereas ROX is due to oxidative side reactions remaining after inhibition of the electron transfer pathway. - »REN«, »ROX«  - communicated by »Timon-Gomez Alba« and »Leo Elettra«

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