Description
DL-Protocols can be selected in DatLab (DL7.1) in the pull-down menu 'Protocol': Set DL-Protocol / O2 limit. A DL-Protocol defines the sequence of Events and Marks. Instrumental DL-Protocols are used for calibrations and instrumental quality control, typically without experimental sample in the incubation medium. DL-Protocols for Substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both. The Titration-Injection-microPump TIP2k can be programmed to control automatically TIP2k-protocols. A collection of evaluated and tested standard DL-Protocols are provided. These can be edited and saved under 'Lab protocols' or under the User logged into DatLab. Moreover, an automatic warning at low oxygen levels can be set to remind the user to re-oxygenate the chamber in time.
Abbreviation: n.a.
MitoPedia O2k and high-resolution respirometry:
DatLab
- Show DL-Protocol can be ckeck-marked to hide or show the DL-Protocol window. Alternatively, the ">>" button (upper left) in the DL-Procotol window can be used to hide or show the window.
- Synchronous DL-Protocol events
- Set DL-Protocol events
- A: Export DL-Protocol
- B: Export DL-Protocol applies for chamber B as described above in 'A: Export DL-Protocol'.
Running a DL-Protocol
- DL-Protocol will inform you if a titration event is missed or a repetition of a non multiple titration ocurred (highlighted in red).
Titrations in SUIT protocols
- Stock concentration of substance B in the titration syringe, cB,in [mM] or [Β΅M] as specified.
- Titration volume, v [Β΅l]
- Chamber volume, V [Β΅l]
- Final concentration, cB [mM] or [Β΅M] as specified.
- cB [mM] = cB,in [mM] x v [Β΅l] / V [Β΅l]
- Example: Malate (M), cM,in = 800 mM stock; v = 5 Β΅l titration volume; V = 2,000 Β΅l chamber volume.
- cM [mM] = cM,in [mM] x v [Β΅l] / V [Β΅l] = 800 mM x 5 Β΅l / 2,000 Β΅l = 2 mM