Search results

...donors: 4 to 5 • Respiration media: MiR05 •Cell type: freshly isolated PLT Protocols (respirometry/Fluorometry): • AmR_SUIT 9_pce: 1S;2D;3P;4Ama • SUIT 7: c == Visiting scientist in the Oroboros O2k-Laboratory ==

[[Image:The world as a laboratory.jpg|right|180px|link=http://wiki.oroboros.at/index.php/The_world_as_a_labor ...Dr.a Isabel Moreno (right) belongs to the physics staff of the Chacaltaya laboratory (Jul/Aug 2012).]]

...days) prior to being saponin permeabilized for HRR using a variety of SUIT protocols on an Oroboros Oxygraph-2k. 1 - Mitochondrial Physiology Laboratory, Department of Health and Exercise Science, Colorado State University, Fort

...Diagnosis of Mitochondrial Defects and Tissue Characterization''' - MiPNet Protocols for High-Resolution Respirometry ...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory (AT)

...rent methods in healthy human volunteers. In addition, we are applying the protocols to a porcine trauma brain injury model, to analyze mitochondrial (dys-)func ...otocols to a porcine TBI model, which we are currently establishing in our laboratory. Our preliminary data suggests that the techniques, established in human PB

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days. ::::» Oroboros O2k-Laboratory

Laboratory of Behavioral Genetics :* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...iable data in commonly used substrate-uncoupler-inhibitor-titration (SUIT) protocols and cyanide titration is needed to obtain precise data for COX excess capac ...xygen sensor were performed with a software package developed for internal laboratory use. In the second part of the study, COX inhibition was tested with sequen

...in many laboratories [1]. Beyond the instrumental level, a standardized mt-laboratory quality management system (QMSmtf [2]) is required for establishing a globa ...ph-2k applying standard substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocols for permeabilized cells in [[MiR05]]. For HRR with intact cells, cyropreser

...with the isolated mitochondria using a conventional oxygraph and standard protocols (performed by Mariana Rosca, M.D. [1]), while for the permeabilized fibers, 2-Daniel Swarovski Research Laboratory, Dept General Transplant Surgery, Med Univ Innsbruck, Austria

...epilept(ogen)ic medication. Therefore, we are in the process of developing protocols to analyze different mitochondrial parameters using the Oxygraph-2K (Orobor ...rtment of Visceral, Transplant and Thoracic Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria

|title=[[Image:DoerrierC.JPG|left|90px|Carolina Doerrier]] Inter-laboratory study on human permeabilized myofibers: effects of fiber preparation, respi ...ented during the MitoEAGLE meeting and next steps will be discussed. Inter-laboratory studies, ring tests, more robust experimental design, and care in statistic

...[3]; 2) a blinded international study measuring simultaneously in the same laboratory respiration of permeabilized human skeletal fibers by high-resolution respi ...er E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. MitoFit Preprint Arch. doi:10.26124/m

...dedicated MitoEAGLE knowledge management network developing harmonization protocols towards generating a rigorously monitored data repository on mitochondrial ...Dept Visceral, Transplant and Thoracic Surgery, Daniel Swarovski Research Laboratory, Medical University Innsbruck, Austria

...y''. Links will be made available to relevant websites, including selected protocols for practical approaches to MiP. In due time, a hard copy will be produced :::<small>Daniel Swarovski Research Laboratory, Department of General and Transplant Surgery, Medical University Innsbruck

...ry OXPHOS control: multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) and enzymatic thresholds. ...ros Instrumetns and Medical University of Innsbruck, D. Swarovski Research Laboratory

:::: National Research Laboratory for Mitochondrial Signaling ::::::* SUIT-Protocols » [[Run DL-Protocol/Set O2 limit]]

|DatLab 7, DL-Protocols. ...nce methods for evaluation of mt-membrane potential. Examples in different protocols.

..., as it offers a large collection of relevant preprints, publications, and protocols, which are always up-to-date and therefore create a platform where recent k ...l in the process of final manuscript preparation, such as harmonization of protocols, which not only help to create clear guidelines for other researchers but a

...om C57BL/6 mice. Respiration media (37 °C) were optimized for the specific protocols. High-resolution respirometry (HRR) was applied with the O2k-Fluorometer (O ...niv, Bratislava, Slovakia; 3-Dept Medical Biochem, Semmelweis Univ, MTA-SE Laboratory Neurobiochem, Budapest, Hungary; 4-D.Swarovski Research Lab, Dept Visceral,