From Bioblast
Description
Abbreviation: RLE(S)
Reference: A CCP(S)_RP2
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: RLE(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
R,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 3Rot,4S;5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (1R;2Omy;3U;4Rot;5S;6Ama).
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).