SUIT-003 O2 ce D028: Difference between revisions
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|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
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Revision as of 15:10, 15 January 2019
Description
Abbreviation: ceS
Reference: A CCP(S)
O2k-Application: O2
- SUIT-category: ceS
- SUIT protocol pattern: linear cell coupling control protocol ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama
ceR,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- ce4Rot,ce5S;ce6Ama
- ce4Rot: In many intact cells ROX measured after titration of rotenone (ce4Rot) is not or only slightly further inhibited by subsequent titration of antimycin A.
- ce5S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
- ce6Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
MitoPedia concepts: SUIT protocol, Recommended
MitoPedia methods:
Respirometry