Amytal: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|description= | |abbr=Amy | ||
|description= Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is and barbiturate drug and an inhibitor of [[Complex I]]. '''TOXIC!''' | |||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | }} | ||
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|type=Inhibitor ETS | |type=Inhibitor ETS | ||
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== Application in [[HRR]] == | |||
'''Amy: Amytal'''ย (Amytal sodium salt,ย C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na);ย Sigma A 4430, 50 g; FW = 248.25 | |||
'''Preparation of 200 mM stock solution''' (dissolved in 99.9% Ethanol): | |||
::1) weigh 0.497 g of Amytal | |||
::2) dissolve in 10 ml 50% ethanol | |||
::3) divide into 500 ยตl aliquots (use dark glass vials - light sensitive) | |||
::4) store frozen at -20 ยฐC | |||
'''Comment:''' | |||
''The product is withdrawn from Sigma but some residual amount is still available.'' |
Revision as of 13:15, 16 May 2012
Description
Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is and barbiturate drug and an inhibitor of Complex I. TOXIC!
Abbreviation: Amy
MitoPedia topics:
Inhibitor
Application in HRR
Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; FW = 248.25
Preparation of 200 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 0.497 g of Amytal
- 2) dissolve in 10 ml 50% ethanol
- 3) divide into 500 ยตl aliquots (use dark glass vials - light sensitive)
- 4) store frozen at -20 ยฐC
Comment:
The product is withdrawn from Sigma but some residual amount is still available.