O2k-Innovation: Difference between revisions
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{{ | {{Oroboros highlights page name}} | ||
:::: <big><big> | :::: <big><big>Tyrolean Government grant</big></big> | ||
[[File:Sponsorlogo gefoerdert RGB.jpg|right|350px]] | [[File:Sponsorlogo gefoerdert RGB.jpg|right|350px]] | ||
* The project ''' | ::::* The project '''O2k-Innovation''' (officially NextGen-O2k project) was supported by the "'''Technologieförderungsprogramm - Tiroler Innovationsförderung'''" of the Tyrolean Government. | ||
* '''Project duration''': 2015-02-01 to 2017-01-31 | ::::* '''Project duration''': 2015-02-01 to 2017-01-31 | ||
* '''Partners''': | ::::* '''[[Partners in the Oroboros Ecosystem]]''': WGT Elektronik, Software Security Networks | ||
* '''Application''': 2015-01-30; '''Granted''': 2015-03-24 (total budget € 350.000) | ::::* '''Application''': 2015-01-30; '''Granted''': 2015-03-24 (total budget € 350.000) | ||
* Funding: '''€ 140,000''' plus a bonus of '''€ 5,000''' for the implementation of gender sensitive actions | ::::* Funding: '''€ 140,000''' plus a bonus of '''€ 5,000''' for the implementation of gender sensitive actions | ||
== | == O2k-Innovation publication == | ||
::: Different stages of prototype development: The | ::::* Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. - [[Harrison_2015_J_Appl_Physiol |»Bioblast link«]] | ||
== The fourth dimension of respiratory control == | |||
::::# On the "line": one dimension is insufficient. | |||
::::# Coupling and pathway control are two necessarily connected dimensions defining the “area” of respiratory control. | |||
::::# Adding kinetics (including substrate kinetics of O<sub>2</sub>, CHO, adenylates, P<sub>i</sub>, and flux-force kinetics) is the third dimension bringing “volume” into the picture of classical mitochondrial respiratory control. | |||
::::# The O2k-Innovation on top of O2k-MultiSensor modules and the [[O2k-FluoRespirometer]] represent the fourth dimension of respiratory control, by adding analysis of functional variables (such as ROS production, mt-membrane potential, Ca<sup>2+</sup>, proton flux, cytochrome and NADH redox states, ATP production) to the measurement of O<sub>2</sub> and O<sub>2</sub>-flux in the same chamber. | |||
== Development of the O2k-Innovation prototype: a look inside == | |||
:::: Different stages of prototype development: The O2k-Innovation is becoming more and more packed with two spectrofluorometers, two spectrophotometers, light guides, Peltier temperatue control with new heat pipes, microelectronics. | |||
<gallery mode=default perrow=2 widths="500px" heights="300px"> | <gallery mode=default perrow=2 widths="500px" heights="300px"> | ||
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File:NextGen_5.JPG | File:NextGen_5.JPG | ||
</gallery> | </gallery> | ||
:::: The '''[[NextGen-O2k]]''' builds upon and extends the concept of the O2k-Fluorometer elaborated within the [[MitoCom_O2k-Fluorometer|K-Regio Project ''MitoCom O2k-Fluorometer'']] (2011-2014). To broaden the specifications of the [[O2k-FluoRespirometer]], the NextGen-O2k combines '''[[high-resolution respirometry]]''' with a [[Q-Module]] and [[NADH-Module]] for RedoxBiology and the [[PB-Module]] to study PhotoBiology (including photosynthesis). It is our ultimate goal to further extend the NextGen-O2k with O2k-Spectrofluorometry (including ratiometric measurements), and O2k-Spectrophotometry (allowing for simultaneous measurement of redox states of the cytochromes ''aa''<sub>3</sub>, ''b'', and ''c''). |
Latest revision as of 07:10, 29 March 2024
O2k-Innovation
- Tyrolean Government grant
- The project O2k-Innovation (officially NextGen-O2k project) was supported by the "Technologieförderungsprogramm - Tiroler Innovationsförderung" of the Tyrolean Government.
- Project duration: 2015-02-01 to 2017-01-31
- Partners in the Oroboros Ecosystem: WGT Elektronik, Software Security Networks
- Application: 2015-01-30; Granted: 2015-03-24 (total budget € 350.000)
- Funding: € 140,000 plus a bonus of € 5,000 for the implementation of gender sensitive actions
O2k-Innovation publication
- Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. - »Bioblast link«
The fourth dimension of respiratory control
- On the "line": one dimension is insufficient.
- Coupling and pathway control are two necessarily connected dimensions defining the “area” of respiratory control.
- Adding kinetics (including substrate kinetics of O2, CHO, adenylates, Pi, and flux-force kinetics) is the third dimension bringing “volume” into the picture of classical mitochondrial respiratory control.
- The O2k-Innovation on top of O2k-MultiSensor modules and the O2k-FluoRespirometer represent the fourth dimension of respiratory control, by adding analysis of functional variables (such as ROS production, mt-membrane potential, Ca2+, proton flux, cytochrome and NADH redox states, ATP production) to the measurement of O2 and O2-flux in the same chamber.
Development of the O2k-Innovation prototype: a look inside
- Different stages of prototype development: The O2k-Innovation is becoming more and more packed with two spectrofluorometers, two spectrophotometers, light guides, Peltier temperatue control with new heat pipes, microelectronics.
- The NextGen-O2k builds upon and extends the concept of the O2k-Fluorometer elaborated within the K-Regio Project MitoCom O2k-Fluorometer (2011-2014). To broaden the specifications of the O2k-FluoRespirometer, the NextGen-O2k combines high-resolution respirometry with a Q-Module and NADH-Module for RedoxBiology and the PB-Module to study PhotoBiology (including photosynthesis). It is our ultimate goal to further extend the NextGen-O2k with O2k-Spectrofluorometry (including ratiometric measurements), and O2k-Spectrophotometry (allowing for simultaneous measurement of redox states of the cytochromes aa3, b, and c).