Coupling-control protocol: Difference between revisions

Revision as of 18:38, 20 April 2014

high-resolution terminology - matching measurements at high-resolution

Coupling-control protocol

Description

[[Description::A phosphorylation control protocol induces different coupling control states at constant substrate supply. In intact cells, the PCP can be applied by using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. CCP). Coupling control states in intact cells include R, L, E; LEAK, ROUTINE, and ETS. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include L, P, E; LEAK, OXPHOS, and ETS.]]

Abbreviation: PCP

Reference: [[Info::Pesta 2012 Methods Mol. Biol.]]

MitoPedia methods: Respirometry

MitoPedia topics: Respiratory state

Biochemical coupling efficiency: from 0 to <1

More details: Biochemical coupling efficiency

Labels:

Regulation: Respiration; OXPHOS; ETS Capacity

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 {{MitoPedia
 |abbr=PCP
-|description=A '''phosphorylation control protocol''' induces different [[coupling control state]]s at constant substrate supply. In [[intact cell]]s, the PCP can be applied by using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[FCCP]]). Coupling control states in intact cells include ''R'', ''L'', ''E''; [[LEAK]], [[ROUTINE]], and [[ETS]]. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include ''L'', ''P'', ''E''; LEAK, [[OXPHOS]], and ETS.
+|description=A '''phosphorylation control protocol''' induces different [[coupling control state]]s at constant substrate supply. In [[intact cell]]s, the PCP can be applied by using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[CCP]]). Coupling control states in intact cells include ''R'', ''L'', ''E''; [[LEAK]], [[ROUTINE]], and [[ETS]]. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include ''L'', ''P'', ''E''; LEAK, [[OXPHOS]], and ETS.
 |info=[[Pesta 2012 Methods Mol. Biol.]]
 |type=Respiration