Sivitz 2017 Neuromethods: Difference between revisions
(Created page with "{{Publication |title=Sivitz WI (2017) Techniques to investigate bioenergetics of mitochondria. In: Strack S, Usachev Y (eds) Techniques to investigate mitochondrial function i...") |
No edit summary |
||
| Line 5: | Line 5: | ||
|year=2017 | |year=2017 | ||
|journal=Neuromethods | |journal=Neuromethods | ||
|abstract= | |abstract=In this chapter we review basic mitochondrial physiology as related to respiration, membrane potential, and ATP production and the relationship to ROS and calcium . Methods for measuring various functional parameters are described. Techniques differ according to whether one is assessing isolated mitochondria or intact cells. Although isolated mitochondria remain the only way to evaluate properties intrinsic to the organelles per se, mitochondrial function clearly is dependent on a multitude of cellular and whole body factors. | ||
In this chapter we review basic mitochondrial physiology as related to respiration, membrane potential, and ATP production and the relationship to ROS and calcium . Methods for measuring various functional parameters are described. Techniques differ according to whether one is assessing isolated mitochondria or intact cells. Although isolated mitochondria remain the only way to evaluate properties intrinsic to the organelles per se, mitochondrial function clearly is dependent on a multitude of cellular and whole body factors. | |||
|editor=Gnaiger E | |editor=Gnaiger E | ||
|mipnetlab=US IA Iowa City Sivitz WI | |mipnetlab=US IA Iowa City Sivitz WI | ||
}} | }} | ||
[[File:Sivitz 2017 Neuromethods CORRECTION.png|right|400px]] | |||
{{Template:Correction FADH2 and S-pathway}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
| Line 32: | Line 18: | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 05:30, 23 October 2023
| Sivitz WI (2017) Techniques to investigate bioenergetics of mitochondria. In: Strack S, Usachev Y (eds) Techniques to investigate mitochondrial function in neurons. Neuromethods 123:67-94. Humana Press, New York. https://doi.org/10.1007/978-1-4939-6890-9_4 |
Sivitz WI (2017) Neuromethods
Abstract: In this chapter we review basic mitochondrial physiology as related to respiration, membrane potential, and ATP production and the relationship to ROS and calcium . Methods for measuring various functional parameters are described. Techniques differ according to whether one is assessing isolated mitochondria or intact cells. Although isolated mitochondria remain the only way to evaluate properties intrinsic to the organelles per se, mitochondrial function clearly is dependent on a multitude of cellular and whole body factors.
• Bioblast editor: Gnaiger E • O2k-Network Lab: US IA Iowa City Sivitz WI
Correction: FADH2 and Complex II
- FADH2 is shown as the substrate feeding electrons into Complex II (CII). This is wrong and requires correction - for details see Gnaiger (2024).
- Gnaiger E (2024) Complex II ambiguities ― FADH2 in the electron transfer system. J Biol Chem 300:105470. https://doi.org/10.1016/j.jbc.2023.105470 - »Bioblast link«
Labels: MiParea: Respiration
Preparation: Isolated mitochondria, Intact cells
Coupling state: LEAK, OXPHOS, ET
Pathway: F, N, S
HRR: Oxygraph-2k
