Talk:Bioblast alert 2015: Difference between revisions

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== Bioblast alert - in preparation ==
== Bioblast editors help ==
Some of the following topics - not more than four per alert - will be selected for a '''Bioblast alert''' and added above under '''next issue in preparation'''. Keep the text short, mainly providing the link to the Bioblast page or to an external link. Important topics may be added into the boxes of the [[Bioblast]] main page, to be included in a Bioblast alert later. See Bioblast alert from previous years to avoid too much repetition.


=== Bioblast alert 2012 (6): 2012-x-x ===
=== Gentle Science ===
[[Image:BB-Bioblast.jpg|left|30px|link=Gentle Science|Gentle Science]] '''Gentle Science''': Β 
* O2k-applications in [[Proc Natl Acad Sci U S A|PNAS]]
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=== Bioblast alert 2012 (5): 2012-06-04 ===
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Zhang J, Nuebel E, Wisidagama DR, Setoguchi K, Hong JS, Van Horn CM, Imam SS, Vergnes L, Malone CS, Koehler CM, Teitell MA (2012) Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells. Nat Protoc 7: 1068-1085. [[Zhang_2012_Nat Protoc]]
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::The O2k in cell culture apllications:
* Pesta D, Gnaiger E (2012) [[Pesta 2012 Methods Mol Biol|High-resolution respirometry. OXPHOSΒ  protocols for human cells and permeabilized fibres from small biopisiesΒ  of human muscle.]] Methods Mol Biol 810: 25-58.
* Garedew A, Haffner B, Huetter E, Gnaiger E.[[MiPNet10.04 CellRespiration|An experiment withΒ  high-resolution respirometry: Phosphorylation control in cellΒ  respiration.]] Mitochondr Physiol Network 10.04.
* Gnaiger E.[[MiPNet11.05 Mitos-PermeabilizedCells|Isolated mitochondria or permeabilized tissues and cells.]] Mitochondr Physiol Network 11.05.
* OROBOROS vs. Seahorse - continue the [[O2k_versus_multiwell_respirometer|DISCUSSION]]
* [http://www.umdf.org/site/c.piKYL1PHLtF/b.4864979/k.C003/Home.htm UMDF: Mitochondrial Medicine 2012] - 2012-June-13 to 16Β  -Β  Capitol Hill, Bethesda, Washington DC, USA
:: === Highlighted MiPNet laboratories - Washington, DC, USA ===
* '''[[US WA Seattle Marcinek DJ|US_WA: Dr. David J Marcinek]] and [[US WA Seattle Kushmerick MJ|US_WA: Dr. Martin J Kushmerick]]'''
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=== Bioblast alert 2012 (##): 2012-##-## ===
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Mitochondrial dys/function in heart vs. brain:
:# WΓΌst RC, Myers DS, Stones R, Benoist D, Robinson PA, Boyle JP, Peers C, White E, Rossiter HB (2011) Regional skeletal muscle remodeling and mitochondrial dysfunction in right ventricular heart failure. Am J Physiol Heart Circ Physiol. 302: H402-11. [[Wuest 2012 Am J Physiol Heart Circ Physiol]].
:# Boyle JP, Hettiarachchi NT, Wilkinson JA, Pearson HA, Scragg JL, Lendon C, Al-Owais MM, Kim CB, Myers DM, Warburton P, Peers C (2012) Cellular consequences of the expression of Alzheimer's disease-causing presenilin 1 mutations in human neuroblastoma (SH-SY5Y) cells. Brain Res Epub ahead of print. [[Boyle 2012 Brain Res]].
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=== MiPNet Laboratory of the week ===
* '''[[UK Leeds Peers C|UK: Prof. Dr. Chris Peers]] and [[Boyle JP|Dr. John Boyle]]'''
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=== Term of the week ===
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Β 
Β 
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=== Bioblast alert ### to select ===
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* Scientific service: Oxygen regime for permeabilized fibres vs. isolated mitochondria - reoxygenations in MiR06.
* Various chemicals:
:''' [[Carboxyatractyloside]] vs. [[atractyloside]]'''
:: ''Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (Β΅M). With Atr, a very high concentration (mM) is required for full inhibition.''
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:: '''Gnaiger E''': When we used atractyloside (Atr), carboxyatractyloside (Cat) was off the market, hence we had no other choice. Cat is available again. Even if it costs more, it is worth more, and with Cat available, I would not bother to find out more about Atr.
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:: '''Chinopoulos C''': I have used the Sigma Cat as well, but not for titration studies. It works perfectly at 1-2 Β΅M concentrations, if you want to block completely all ANTs. The MW is 770.8.
::The Sigma Cat originates from Atr extracted from Xanthicum sibericum. A carboxylation is a further chemical modification. The Merck Cat originates from Atr from Atractylis gummifera, which is identical to that from Xanthicum. A carboxylation is a further chemical modification. Because of this modification which is man-made, there may be different potencies. For titration studies (0-200 nM Cat), the one from Merck (Atractylis gummifera) is strongly recommended. - Christos (2012-04-19)
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::'''Chu MJJ''': Unfortunately Merck does not sell the Cat from Atractylis gummifera anymore and currently only offers the Cat extracted from Xanthicum sibericum (as does Sigma). - Michael (2012-04-20)
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: '''Alpha-glycerophosphate'''
:: DL-alpha-glycerophosphate disodium salt hexahydrate (FW= 324.1), Sigma G-2138
:: Sigma is not selling alpha-glycerophosphate any more!
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== Bioblast alert - concept ==


Here is a brief summary on the concept of '''Bioblast alert'''.
=== MitoPedia: high-resolution terminology ===
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* [[MitoPedia: Respiratory states|high-resolution terminology]]''' - matching measurement at high-resolution.
Some of the following topics (not more than four per alert) will be selected for a '''Bioblast alert'''.
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=== MiPNet O2k-Publications ===
* Topic: [[O2-kinetics]]; [[coupling]]; pathology; ...
* Preparation: [[Homogenate]]/[[isolated mitochondria]]; intact/permeabilized [[cells]]; [[permeabilized fibre]]/in vivo
* Protocols
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=== MiPNet Laboratories of the World ===
Contact the [[MiPNet]] laboratories: show link on the wiki, ask for updates on group, references, website, perhaps major projects ([[HypoxiaNet]]), perhaps add pictures, perhaps keywords/short description.
Circular: link to presented lab / special occasion (e.g. publication; conference presentation; new member; PhD thesis, ..
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=== High-resolution terminology ===
*[[List of respiratory states|high-resolution terminology]]''' - matching measurement at high-resolution. Term of the week: '''[[Basal respiration]]'''
* Define – Measure: terminology and experimental approach
* Define – Measure: terminology and experimental approach
* Define – Clarify: consider discrepancies, resolve problems
* Define – Clarify: consider discrepancies, resolve problems


=== MiP''events'' ===
=== MitoGlobal ===
* MiP''events''
* Conferences and Workshops
* Conferences and Workshops


=== Bioblast ===
=== Bioblast news ===
* What is new or updated on the website: Lists (chemicals, protocols, scientific service, ..)
* What is new or updated on the website: Lists (chemicals, protocols, scientific service, ..). AΒ  term may be selected (perhaps related to the publication), updated, discussed in our group, and then presented in the Bioblast alert. As Bioblast is updated, the updated β€˜article’ can be added to the prepared points for a future Bioblast alert.
* Projects in mitochondrial science (COST [[HypoxiaNet]]; Framework programs; ..)
* Projects in mitochondrial science (COST [[HypoxiaNet]]; Framework programs; ..)
* Links on the Bioblast website; networking in mitochondrial science, presentation of: MIG List, Mitochondrion journal and website; Mitochondrial Societies
* Links on the Bioblast website; networking in mitochondrial science, presentation of: MIG List, Mitochondrion journal and website; Mitochondrial Societies; ..
* MitoCom News


=== Bioblast editors ===
=== Bioblast alert at the turn of the year ===
Example: from 2015 to 2016
# Move the page [[Bioblast alert 2015]] to '''Bioblast alert 2016'''.
# Copy the contents of the new page '''Bioblast alert 2016''' into the old page [[Bioblast alert 2015]]. Delete sections unrelated to 2015; use a previous page ([[Bioblast alert 2014]]) as a template.
# Edit the contents of the new page '''Bioblast alert 2016''': delete the entries from the previous year, add under 'Bioblast alert - previous issues' a link to the previous year ([[Bioblast alert 2015]]).
# Edit the REDIRECT on [[Bioblast alert]] from [[Bioblast alert 2015]] to '''Bioblast alert 2016'''.
# Edit the main page [[Bioblast]], updating the header '''Bioblast alert 2016(01): 2016-0#-##'''.


A [[Bioblast editorial board|Bioblast editor]] may select an O2k-Publication (or a small number of related publications), particularly from a MiPNet Reference Laboratory, may get in contact with the particular reference lab, update info on the lab, and β€˜present’ the lab in the alert. AΒ  term may be selected (perhaps related to the publication), updated, discussed in our group, and then presented in the alert. An editor does not even have to present an entire alert, but may prepare individual β€˜points’, which then can be added to a particular alert. As Bioblast is updated, the updated β€˜article’ (term, publication etc) can be added to the prepared points for a future alert.
=== Not included in Bioblast alert ===
The following topics are not 'Bioblast alert', since they may be considered too close to advertising:
# MiPNet O2k-Publications: A [[Bioblast editorial board|Bioblast editor]] may select an O2k-Publication (or a small number of related publications), particularly from a MiPNet Reference Laboratory, may get in contact with the particular reference lab, update info on the lab, and β€˜present’ the lab on the start page of the Bioblast website. Β 
# MiPNet Laboratories of the World: Contact the [[MiPNet]] laboratories: show link on the wiki, ask for updates on group, references, website, perhaps major projects ([[HypoxiaNet]]), perhaps add pictures, perhaps keywords/short description.
Circular: link to presented lab / special occasion (e.g. publication; conference presentation; new member; PhD thesis, ..
Β 
[[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]
== Popular Bioblast page ==
[[Bioblast alert]] has been accessed more than
::::* 35,000 times (2019-12-11)
::::* 30,000 times (2018-08-01)
::::* 25,000 times (2015-10-28)
::::* 20,000 times (2014-12-31)
::::* 15,000 times (2014-06-12)
::::* 10,000 times (2013-08-18)
::::* 5,000 times (2012-10-22)

Latest revision as of 16:20, 11 December 2019

file=

Bioblast editors help

Some of the following topics - not more than four per alert - will be selected for a Bioblast alert and added above under next issue in preparation. Keep the text short, mainly providing the link to the Bioblast page or to an external link. Important topics may be added into the boxes of the Bioblast main page, to be included in a Bioblast alert later. See Bioblast alert from previous years to avoid too much repetition.

Gentle Science

Gentle Science

Gentle Science:

MitoPedia: high-resolution terminology

  • high-resolution terminology - matching measurement at high-resolution.
  • Define – Measure: terminology and experimental approach
  • Define – Clarify: consider discrepancies, resolve problems

MitoGlobal

  • MiPevents
  • Conferences and Workshops

Bioblast news

  • What is new or updated on the website: Lists (chemicals, protocols, scientific service, ..). A term may be selected (perhaps related to the publication), updated, discussed in our group, and then presented in the Bioblast alert. As Bioblast is updated, the updated β€˜article’ can be added to the prepared points for a future Bioblast alert.
  • Projects in mitochondrial science (COST HypoxiaNet; Framework programs; ..)
  • Links on the Bioblast website; networking in mitochondrial science, presentation of: MIG List, Mitochondrion journal and website; Mitochondrial Societies; ..

Bioblast alert at the turn of the year

Example: from 2015 to 2016

  1. Move the page Bioblast alert 2015 to Bioblast alert 2016.
  2. Copy the contents of the new page Bioblast alert 2016 into the old page Bioblast alert 2015. Delete sections unrelated to 2015; use a previous page (Bioblast alert 2014) as a template.
  3. Edit the contents of the new page Bioblast alert 2016: delete the entries from the previous year, add under 'Bioblast alert - previous issues' a link to the previous year (Bioblast alert 2015).
  4. Edit the REDIRECT on Bioblast alert from Bioblast alert 2015 to Bioblast alert 2016.
  5. Edit the main page Bioblast, updating the header Bioblast alert 2016(01): 2016-0#-##.

Not included in Bioblast alert

The following topics are not 'Bioblast alert', since they may be considered too close to advertising:

  1. MiPNet O2k-Publications: A Bioblast editor may select an O2k-Publication (or a small number of related publications), particularly from a MiPNet Reference Laboratory, may get in contact with the particular reference lab, update info on the lab, and β€˜present’ the lab on the start page of the Bioblast website.
  2. MiPNet Laboratories of the World: Contact the MiPNet laboratories: show link on the wiki, ask for updates on group, references, website, perhaps major projects (HypoxiaNet), perhaps add pictures, perhaps keywords/short description.

Circular: link to presented lab / special occasion (e.g. publication; conference presentation; new member; PhD thesis, ..


Bioblast wiki

Popular Bioblast page

Bioblast alert has been accessed more than

  • 35,000 times (2019-12-11)
  • 30,000 times (2018-08-01)
  • 25,000 times (2015-10-28)
  • 20,000 times (2014-12-31)
  • 15,000 times (2014-06-12)
  • 10,000 times (2013-08-18)
  • 5,000 times (2012-10-22)
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