Rocha 2010 Nitric Oxide: Difference between revisions
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{{Publication | {{Publication | ||
|title=Rocha BS, Gago B, Barbosa RM, Laranjinha J (2010) Diffusion of nitric oxide through the gastric wall upon reduction of nitrite by red wine: physiological impact. | |title=Rocha BS, Gago B, Barbosa RM, Laranjinha J (2010) Diffusion of nitric oxide through the gastric wall upon reduction of nitrite by red wine: physiological impact. Nitric Oxide 22:235-41. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed | |info=[http://www.ncbi.nlm.nih.gov/pubmed/20083218 PMID:20083218] | ||
|authors=Rocha BS, Gago B, Barbosa RM, Laranjinha J | |authors=Rocha BS, Gago B, Barbosa RM, Laranjinha J | ||
|year=2010 | |year=2010 | ||
|journal=Nitric Oxide | |journal=Nitric Oxide | ||
|abstract=In this work we showed that nitric oxide produced via red wine- and ascorbate-dependent reduction of nitrite diffuses through the rat stomach, inducing smooth muscle relaxation. The studies encompassed ex vivo and in vivo models of diffusion. Regarding the former, luminal *NO generated from a mixture of physiologic nitrite and ascorbate or wine diffuses across the stomach wall, being 8-20% of that produced in the mucosal side detected at high microM range (>100 microM) in the serosal side. In order to evaluate whether cellular dysfunction was associated with *NO diffusion at the microM range, the gastric tissue exposed to *NO was evaluated in terms of carbachol-induced muscle contraction in fundal strips and mitochondrial respiration and showed to remain functional and metabolically active. Moreover, pre-contracted gastric strips were shown to relax 86.5+/-5.5% (control) and 75.0+/-4.0% (nitrite/ascorbate-exposed tissue) when challenged with acidified nitrite. The studies in the living animal support the diffusion of luminal *NO to the gastric vasculature as, following addition of nitrite/ascorbate to rat stomach in vivo, *NO was not detected in the serosal environment but concentrations as high as 31 microM of *NO were detected outside the stomach after cardiac arrest. Collectively, the results establish a link between the consumption of nitrite and dietary reductants (e.g., wine polyphenols) and stomach muscle relaxation via the local chemical generation of *NO. | |abstract=In this work we showed that nitric oxide produced via red wine- and ascorbate-dependent reduction of nitrite diffuses through the rat stomach, inducing smooth muscle relaxation. The studies encompassed ''ex vivo'' and ''in vivo'' models of diffusion. Regarding the former, luminal *NO generated from a mixture of physiologic nitrite and ascorbate or wine diffuses across the stomach wall, being 8-20% of that produced in the mucosal side detected at high microM range (>100 microM) in the serosal side. In order to evaluate whether cellular dysfunction was associated with *NO diffusion at the microM range, the gastric tissue exposed to *NO was evaluated in terms of carbachol-induced muscle contraction in fundal strips and mitochondrial respiration and showed to remain functional and metabolically active. Moreover, pre-contracted gastric strips were shown to relax 86.5+/-5.5% (control) and 75.0+/-4.0% (nitrite/ascorbate-exposed tissue) when challenged with acidified nitrite. The studies in the living animal support the diffusion of luminal *NO to the gastric vasculature as, following addition of nitrite/ascorbate to rat stomach ''in vivo'', *NO was not detected in the serosal environment but concentrations as high as 31 microM of *NO were detected outside the stomach after cardiac arrest. Collectively, the results establish a link between the consumption of nitrite and dietary reductants (e.g., wine polyphenols) and stomach muscle relaxation via the local chemical generation of *NO. | ||
|keywords= | |keywords=Nitric oxide, Nitrite, stomach, Diffusion, Polyphenols, Relaxation | ||
|mipnetlab=PT Coimbra Laranjinha J | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
| | |additional=stomach | ||
}} | }} | ||
Latest revision as of 14:55, 27 March 2018
| Rocha BS, Gago B, Barbosa RM, Laranjinha J (2010) Diffusion of nitric oxide through the gastric wall upon reduction of nitrite by red wine: physiological impact. Nitric Oxide 22:235-41. |
Rocha BS, Gago B, Barbosa RM, Laranjinha J (2010) Nitric Oxide
Abstract: In this work we showed that nitric oxide produced via red wine- and ascorbate-dependent reduction of nitrite diffuses through the rat stomach, inducing smooth muscle relaxation. The studies encompassed ex vivo and in vivo models of diffusion. Regarding the former, luminal *NO generated from a mixture of physiologic nitrite and ascorbate or wine diffuses across the stomach wall, being 8-20% of that produced in the mucosal side detected at high microM range (>100 microM) in the serosal side. In order to evaluate whether cellular dysfunction was associated with *NO diffusion at the microM range, the gastric tissue exposed to *NO was evaluated in terms of carbachol-induced muscle contraction in fundal strips and mitochondrial respiration and showed to remain functional and metabolically active. Moreover, pre-contracted gastric strips were shown to relax 86.5+/-5.5% (control) and 75.0+/-4.0% (nitrite/ascorbate-exposed tissue) when challenged with acidified nitrite. The studies in the living animal support the diffusion of luminal *NO to the gastric vasculature as, following addition of nitrite/ascorbate to rat stomach in vivo, *NO was not detected in the serosal environment but concentrations as high as 31 microM of *NO were detected outside the stomach after cardiac arrest. Collectively, the results establish a link between the consumption of nitrite and dietary reductants (e.g., wine polyphenols) and stomach muscle relaxation via the local chemical generation of *NO. • Keywords: Nitric oxide, Nitrite, stomach, Diffusion, Polyphenols, Relaxation
• O2k-Network Lab: PT Coimbra Laranjinha J
Labels:
Organism: Rat
HRR: Oxygraph-2k
stomach
